Western Blotting (WB), Immunohistochemistry (IHC), ELISA
Réactivité croisée
Souris, Rat (Rattus)
Attributs du produit
Concentration Definition: by UV absorbance at 280 nm
Immunogène
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y694 of mouse STAT5a protein.
STAT5A
Reactivité: Humain, Souris, Rat, Chien
WB, IHC, IF, IP
Hôte: Souris
Monoclonal
89-Stat5
unconjugated
Indications d'application
This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 91 kDa in size corresponding to phosphorylated Stat5a protein by western blotting in the appropriate cell lysate or extract. This phospho-specific polyclonal antibody reacts with mouse Stat5a pY694 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.
Restrictions
For Research Use only
Format
Liquid
Concentration
1.1 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
-20 °C
Antigène
STAT5A
(Signal Transducer and Activator of Transcription 5A (STAT5A))
anticorps MGF, anticorps STAT5, anticorps AA959963, anticorps Stat5, anticorps STAT5A/MGF, anticorps STATA5, anticorps STAT5A, anticorps stat5, anticorps stat5b, anticorps signal transducer and activator of transcription 5A, anticorps signal transducer and activator of transcription 5a, anticorps STAT5A, anticorps Stat5a, anticorps stat5a, anticorps LOC100348518, anticorps LOC100716811
Sujet
This antibody is designed, produced, and is suitable for Cancer, Immunology and Nuclear Signaling research. Signal trans-ducer and activator of transcription 5 (Stat5) belongs to a family of cytoplasmic trans-cription factors that can be activated (phosphorylated) by a cell surface receptor. Phosphorylation at Tyr694 is obligatory for Stat5 activation. Stat5 has two isoforms, Stat5a and Stat5b. Aberrant Stat5 activation has been implicated in the pathogenesis of chronic myelogenous leukemia, prostate and breast cancer and tumor metastasis. Stat5 is localized in the cytoplasm and upon phosphorylation at Y694 is translocated to the nucleus. Synonyms: MGF antibody, Signal Transducer and Activator of Transcription 5A antibody, STAT 5A antibody, Transcription factor STAT5A antibody