Growth Hormone 1 anticorps

N° du produit ABIN235517

L’anticorps Souris Monoclonal anti-Growth Hormone 1 a été validé pour ELISA et a été validé indépendamment. Il convient pour détecter Growth Hormone 1 dans des échantillons de Humain.

Aperçu rapide pour Growth Hormone 1 anticorps (ABIN235517)

Antigène: Growth Hormone 1 (GH1)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Growth Hormone 1 est non-conjugé

Application: ELISA

Clone: 090-10154

Détail du produit anti-Growth Hormone 1 anticorps

Réactivité croisée (Details): Cross-reactivity: Synthetic hGH (Somatonorm) 100 % Human Placental Lactogen <0.00 % Human Prolactin <0.00 %

Attributs du produit: MAb to hGH, Monoclonal antibody to Human Growth Hormone (hGH)

Purification: Protein A Chromatography. Ascites

Stérilité: 0.2 μm filtered

Immunogène: hGH isolated from human pituitary gland

Isotype: IgG1

Information d'application

Indications d'application: Suitable for use in ELISA. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Restrictions: For Research Use only

Independent Validation (UP)

Validation #102719 (Unfolding Profile)

by: NanoTemper Technologies

No.: #102719

Date: 05.11.2018

Antigène: GH1

Numéro du lot: H0833.1

Application validée: Unfolding Profile

Contrôle positif: ABIN235517

Conclusion:

Passed. ABIN235517 showed a clear unfolding profile with one detected unfolding event with a T_i at 76.3°C. This suggests that the protein is properly folded and functional.

Validation Images

Unfolding profile of ABIN235517. The fluorescence signal is plotted against temperature. The native (folded) protein has a low signal at the beginning of the experiment, which increases upon unfolding, showing one unfolding event. The vertical line indicates the Ti at 76.3°C.

Protocole

Anticorps primaire: ABIN235517

Full Protocol:

• Dilute ABIN235517 1:10 in PBS buffer (Roth, 1058.1, lot 285231988) to get a final volume of 15µl at a concentration of 3.33µM.
• Load sample into Tycho capillary (NanoTemper Technologies, TY-C001).
• Run Tycho measurement.

Notes:

Tycho is designed to run quick and precise protein quality check experiments. Tycho uses intrinsic protein fluorescence to follow protein unfolding while running a fast thermal ramp, yielding results in 3min. A protein’s unfolding behavior is characterized by various parameters, most notably the inflection temperature (T_i). The T_i can be used to identify properly folded protein, to compare different batches, or to analyze the influence of storage/transport conditions on a protein. An absence of T_i would suggest that the protein is already unfolded and therefore most likely nonfunctional.

Stockage

Format: Liquid

Buffer: 10 mM Phosphate, pH 7.4 containing 150 mM Sodium chloride, 0.1 % Sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles.
Centrifuge product if not completely clear after standing at room temperature.
Prepare working dilution only prior to immediate use.

Stock: 4 °C/-80 °C

Stockage commentaire: Short term (up to 7 days) store at 2-8 °C. Long term, aliquot and store at <-48 °C. If aliquoted for long term storage, fill volume should be equal to or greater than 50 % of the nominal fill volume of the vial used.

Date de péremption: 1 week

Détails sur Growth Hormone 1

Antigène: Growth Hormone 1 (GH1)

Autre désignation: Growth Hormone (hGH)

Pathways: Signalisation NF-kappaB, Signalistation JAK/STAT, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Response to Growth Hormone Stimulus, Regulation of Hormone Biosynthetic Process