WISP1 anticorps (C-Term)

N° du produit ABIN238610

L’anticorps Chèvre Polyclonal anti-WISP1 a été validé pour WB, ELISA, IF et FACS. Il convient pour détecter WISP1 dans des échantillons de Humain.

Aperçu rapide pour WISP1 anticorps (C-Term) (ABIN238610)

Antigène: WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp WISP1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Classe de qualité: Verified

Détail du produit anti-WISP1 anticorps

Épitope: C-Term

Fonction: WISP1

Séquence: ESYPDFSEIA N

Specificité: This antibody is expected to recognize both reported isoforms (NP_003873.1, NP_543028.1).

Réactivité croisée: Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-ESYPDFSEIAN, from the C Terminus of the protein sequence according to NP_003873.1, NP_543028.1.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 32-35 kDa band observed in lysates of cell line CaCo-2 and approx. 40 kDa in Human Tonsil lysates, and additionally in a lysate of cell line CaCo-2, which were successfully blocked by incubation with the immunising peptide (calculated

Peptide ELISA: antibody detection limit dilution 1:128000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur WISP1

Antigène: WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))

Autre désignation: CCN4

Sujet: WISP1, WNT1 inducible signaling pathway protein 1, CCN4, WISP1c, WISP1i, WISP1tc, WNT1 induced secreted protein 1, Wnt-1 inducible signaling pathway protein 1, wnt-1 signaling pathway protein 1

ID gène: 8840, 22402, 65154

NCBI Accession: NP_003873, NP_543028

Pathways: Signalisation WNT, Growth Factor Binding