ABCB5 anticorps (Internal Region)

N° du produit ABIN238622

L’anticorps Chèvre Polyclonal anti-ABCB5 a été validé pour WB, ELISA et FACS. Il convient pour détecter ABCB5 dans des échantillons de Humain. Il y a 1 publication disponible.

Aperçu rapide pour ABCB5 anticorps (Internal Region) (ABIN238622)

Antigène: ABCB5 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 5 (ABCB5))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp ABCB5 est non-conjugé

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Classe de qualité: Verified

Détail du produit anti-ABCB5 anticorps

Épitope: Internal Region

Fonction: ABCB5

Séquence: QTQHRNTSKK AQ

Specificité: This antibody is expected to recognise both reported isoforms (NP_848654.3, NP_001157413.1)

Réactivité croisée: Humain

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-QTQHRNTSKKAQ , from the internal region of the protein sequence according to NP_848654.3, NP_001157413.1.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 80 kDa band observed in lysates of cell line A431(calculated MW of 89.8 kDa according to NP_848654.3). Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:32000.

Commentaires:

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-ABCB5 (ABIN238622)

Cheung, Cheung, Cheng, Wong, Fan: "Granulin-epithelin precursor and ATP-dependent binding cassette (ABC)B5 regulate liver cancer cell chemoresistance." dans: Gastroenterology, Vol. 140, Issue 1, pp. 344-55, (2010) (PubMed).

Détails sur ABCB5

Antigène: ABCB5 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 5 (ABCB5))

Autre désignation: ABCB5

Sujet: ATP-binding cassette, sub-family B (MDR/TAP), member 5 , ABCB5alpha, ABCB5beta, EST422562, ATP-binding cassette, sub-family B, member 5N: P-glycoprotein ABCB5

Poids moléculaire: 89.8kDa

ID gène: 340273

NCBI Accession: NP_848654, NP_001157413