RFT1 anticorps

N° du produit ABIN2459371

Détail du produit anti-RFT1 anticorps

Antigène: RFT1 (RFT1 Homolog (RFT1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RFT1 est non-conjugé

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogène: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RFT1.

Information d'application

Indications d'application: RFT1 antibody can be used for detection of RFT1 by ELISA at 1:1562500. RFT1 antibody can be used for detection of RFT1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Conseil sur la manipulation: As with any antibody avoid repeat freeze-thaw cycles.

Stock: 4 °C/-20 °C

Stockage commentaire: For short periods of storage (days) store at 4 °C. For longer periods of storage, store RFT1 antibody at -20 °C.

Détails sur RFT1

Antigène: RFT1 (RFT1 Homolog (RFT1))

Autre désignation: RFT1 (RFT1 Produits)

Synonymes: anticorps GB14370, anticorps CDG1N, anticorps D930025H04, anticorps RGD1562654, anticorps protein RFT1 homolog, anticorps RFT1 homolog, anticorps LOC412489, anticorps LOC100453537, anticorps RFT1, anticorps rft1, anticorps LOC100633251, anticorps LOC100651701, anticorps Rft1

Sujet: N-glycosylation of proteins follows a highly conserved pathway that begins with the synthesis of a Man (5)GlcNAc (2)-dolichylpyrophosphate (PP-Dol) intermediate on the cytoplasmic side of the endoplasmic reticulum (ER) membrane followed by the translocation of Man (5)GlcNAc (2)-PP-Dol to the luminal side of the ER membrane. RFT1 is the flippase enzyme that catalyzes this translocation.N-glycosylation of proteins follows a highly conserved pathway that begins with the synthesis of a Man (5)GlcNAc (2)-dolichylpyrophosphate (PP-Dol) intermediate on the cytoplasmic side of the endoplasmic reticulum (ER) membrane followed by the translocation of Man (5)GlcNAc (2)-PP-Dol to the luminal side of the ER membrane. RFT1 is the flippase enzyme that catalyzes this translocation (Helenius et al., 2002 [PubMed 11807558]).

Poids moléculaire: 60 kDa

ID gène: 91869

NCBI Accession: NP_443091

UniProt: Q96AA3