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POLR3B anticorps

Cet anticorps anti-POLR3B est un anticorps Lapin Polyclonal détectant POLR3B dans WB et ELISA. Adapté pour Humain, Souris, Rat et Drosophila melanogaster.
N° du produit ABIN2463219

Aperçu rapide pour POLR3B anticorps (ABIN2463219)

Antigène

Voir toutes POLR3B Anticorps
POLR3B (Polymerase (RNA) III (DNA Directed) Polypeptide B (POLR3B))

Reactivité

  • 27
  • 22
  • 8
  • 4
  • 4
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Humain, Souris, Rat, Drosophila melanogaster

Hôte

  • 32
  • 2
Lapin

Clonalité

  • 32
  • 2
Polyclonal

Conjugué

  • 18
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp POLR3B est non-conjugé

Application

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  • 4
  • 2
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  • 1
  • 1
Western Blotting (WB), ELISA
  • Purification

    Antibody is purified by peptide affinity chromatography method.

    Immunogène

    Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human POLR3B.
  • Indications d'application

    POLR3B antibody can be used for detection of POLR3B by ELISA at 1:12500. POLR3B antibody can be used for detection of POLR3B by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

    Concentration

    1 mg/mL

    Buffer

    Antibody is lyophilized in PBS buffer with 2 % sucrose.

    Conseil sur la manipulation

    As with any antibody avoid repeat freeze-thaw cycles.

    Stock

    4 °C/-20 °C

    Stockage commentaire

    For short periods of storage (days) store at 4 °C. For longer periods of storage, store POLR3B antibody at -20 °C.
  • Antigène

    POLR3B (Polymerase (RNA) III (DNA Directed) Polypeptide B (POLR3B))

    Autre désignation

    POLR3B

    Sujet

    POLR3B belongs to the RNA polymerase beta chain family. DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. POLR3B is the second largest core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. It is proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol III is composed of mobile elements and RPC2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft.

    Poids moléculaire

    128 kDa

    ID gène

    55703

    NCBI Accession

    NP_060552

    UniProt

    Q9NW08
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