BTK anticorps (truncated)

N° du produit ABIN2464023

Cet anticorps anti-BTK Monoclonal Souris (Clone 7F12H4) (ABIN2464023) détecte spécifiquement BTK dans WB, ELISA et IHC. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour BTK anticorps (truncated) (ABIN2464023)

Antigène: BTK (Bruton Agammaglobulinemia tyrosine Kinase (BTK))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp BTK est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Clone: 7F12H4

Détail du produit anti-BTK anticorps

Épitope: truncated

Immunogène: Ni-NTA purified truncated recombinant BTK expressed in E. Coli strain BL21 (DE3).

Isotype: IgG1

Information d'application

Indications d'application: Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Stock: -20 °C

Stockage commentaire: BTK monoclonal antibody can be stored at -20 °C, stable for one year.

Détails sur BTK

Antigène: BTK (Bruton Agammaglobulinemia tyrosine Kinase (BTK))

Autre désignation: BTK

Sujet: Brutons tyrosine kinase (BTK) is a member of the BTK/Tec family of cytoplasmic tyrosine kinases.All members of the family contain SH3 and SH2 domains and, with the exception of Txk and Dsrc28C, also contain a pleckstrin homology (PH) and a Tec homology (TH) domain in their amino termini.BTK plays an important role in B cell development. Activation of B cells by various ligands is accompanied by BTK membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate. The membrane located BTK is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinase, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for full activation.

ID gène: 695

UniProt: Q06187

Pathways: Fc-epsilon Receptor Signaling Pathway, Hormone Transport, Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, BCR Signaling