Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Purification
IREB2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogène
IREB2 antibody was raised against a 15 amino acid synthetic peptide near the internal region of IREB2.
ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 110 kDa band observed in human liver lysates (calculated MW of 105 kDa according to NP_004127.1). Recommended concentration: 1-3 μg/mL. an additional band of unknown identity was also consistently observed at 60 kDa . This band was successfully blocked by incubation with the immunizing peptide.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Minimize freezing and thawing.
Stock
-20 °C
Stockage commentaire
Aliquot and store at -20 °C.
Antigène
IREB2
(Iron-Responsive Element Binding Protein 2 (IREB2))