This antibody is expected to recognize both reported isoforms (NP_005210.3, NP_001073280.1).
Purification
DIAPH1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogène
DIAPH1 antibody was raised against an 11 amino acid synthetic peptide near the internal region of DIAPH1.
ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 140 kDa band observed in lysates of cell line NIH3T3 (calculated MW of 141 kDa according to human NP_005210.3 and 139 kDa according to mouse NP_031884.1). Recommended concentration: 0.3-1 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.