SLC12A3 anticorps (AA 74-95) (Biotin)

N° du produit ABIN2486292

Détail du produit anti-SLC12A3 anticorps (Biotin)

Antigène: SLC12A3 (Solute Carrier Family 12 (Sodium/Chloride Transporters), Member 3 (SLC12A3))

Épitope: AA 74-95

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SLC12A3 est conjugé à/à la Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoelectron Microscopy (IEM)

Specificité: Detects ~160 kDa.

Réactivité croisée: Chien, Humain, Souris, Rat

Purification: Affinity Purified

Immunogène: Produced against a synthetic peptide mapping to a segment of rat NCC (amino acids 74-95), N-terminal

Information d'application

Indications d'application:

• WB (1:1000)
• IHC (1:200)
• optimal dilutions for assays should be determined by the user.

Commentaires:

1 μg/ml of ABIN2486292 was sufficient for detection of NCC3 in 10 μg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Conjugated antibodies should be stored at 4°C

Détails sur SLC12A3

Antigène: SLC12A3 (Solute Carrier Family 12 (Sodium/Chloride Transporters), Member 3 (SLC12A3))

Autre désignation: NCC (SLC12A3 Produits)

Synonymes: anticorps SLC12A3, anticorps slc12a3, anticorps DKFZp469N2315, anticorps NCC, anticorps NCCT, anticorps TSC, anticorps AI035291, anticorps solute carrier family 12 member 3, anticorps solute carrier family 12 (sodium/chloride transporter), member 3, anticorps solute carrier family 12, member 3, anticorps SLC12A3, anticorps slc12a3.2, anticorps Slc12a3

Sujet: NCC, a thiazide-sensitive NaCl co-transporter, is found on the apical membrane of the distal convoluted tubule, where it is the principal mediator of Na+ and Cl- reabsorption in this segment of the nephron. It is activated by phosphorylation, and has been implicated in renal NaCl and K+ homeostasis (1). Regulation of NCC expression and phosphorylation by dietary NACl restriction appears to involve SGK1(1). In experiments with angiotensin II-infused mice, increased sensitivity to Ang II may involved over-activity of NCC (2). Therefore, NCC is the target of thiazide diuretics used in the treatment of hypertension (1). Molecular experiments have also shown that NCC has been detected in the lens cortex, core and fiber cells of a rat (3).

ID gène: 54300, 6559

NCBI Accession: NP_062218, NP_000330

UniProt: P55018, P55017