HSP70 anticorps (Heat Shock Protein 70) (full length) (Atto 594)

Details for Product anti-HSP70 Antibody No. ABIN2486666, Fournisseur: Connectez-vous pour afficher
Antigène
  • APG-2
  • HS24/P52
  • HSPH2
  • RY
  • hsp70
  • hsp70RY
  • CG31354
  • HSP70
  • Hsp70Bb
  • hsp70B
  • hsp70Bb-prime
  • DmelCG5834
  • CG5834
  • HSPA1
  • HSP70B'
  • HSPA6
  • ARABIDOPSIS HEAT SHOCK PROTEIN 70
  • ATHSP70
  • heat shock protein 70
  • LOC100305036
  • hsc70
  • Hsp70
  • Hsp70-1
  • Hsp70.1
  • hsp68
  • Hsp110
  • irp94
  • HSP70-2
  • HSPA1B
  • HSPA2
  • hsp70-5
  • heat shock protein family A (Hsp70) member 4
  • CG5834 gene product from transcript CG5834-RA
  • heat shock protein 70
  • heat shock protein family A (Hsp70) member 6
  • heat shock 70kDa protein 2
  • heat shock 70 kD protein cognate
  • Hsp70 family chaperone
  • Heat shock protein 70
  • Heat shock protein 70, putative
  • heat shock protein 1B
  • heat shock protein family A member 4
  • heat shock 70kDa protein 1A
  • heat shock protein 1
  • Heat Shock Protein
  • heat shock cognate 70-kd protein
  • HSPA4
  • Hsp70Bbb
  • HSP70
  • HSPA6
  • HSPA2
  • PCC7424_2419
  • Isop_1041
  • CGB_C3390W
  • Bacsa_1698
  • dnaK-B
  • LOC100305036
  • Hspa1b
  • Hspa4
  • HSPA1A
  • hsp1
  • hsp-70
  • hsp70
Épitope
full length
61
52
52
36
36
31
21
19
19
17
15
15
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivité
Beluga, Carp (Cyprinus carpio), Corail, Boeuf (Vache), Chien, Poisson, Cobaye, Hamster, Humain, Leishmania amazonensis, Singe, Souris, Porc, Plantes, Rat (Rattus), Requin, Mouton
836
636
634
286
257
253
238
219
171
164
134
132
126
120
119
89
71
68
58
56
53
42
38
37
37
35
35
26
18
18
17
8
8
6
5
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Hôte
Lapin
482
405
58
36
36
Clonalité
Polyclonal
Conjugué
Cet anticorp HSP70 est conjugé à/à la Atto 594
51
51
40
38
38
36
35
35
35
35
35
35
35
35
35
34
26
9
7
6
5
5
5
5
5
5
4
4
4
3
3
2
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
928
417
404
309
305
294
207
148
145
72
68
62
36
23
11
9
2
1
1
Options
Fournisseur
Connectez-vous pour afficher
N° du produit (Fournisseur)
Connectez-vous pour afficher
Immunogène Full length protein HSP70
Specificité Detects a ~70 kDa. May cross-react with HSC70 at lower dilutions.
Purification Peptide Affinity Purified
Plasmids, Primers & others Plasmids, Primers & others HSP70 products on genomics-online (e.g. as negative or positive controls)
Antigène
Autre désignation HSP70 (HSP70 Antibody Extrait)
Sujet HSP70 genes encode abundant heat-inducible 70- kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50 % identity (1, 2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATPbinding domain displays multiple features of nucleotide binding proteins (5). All HSP70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the HSP70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of HSP70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport. Looking for more information on HSP70? Visit our new HSP70 Scientific Resource Guide at http://www.HSP70.com.
ID gène 3303
NCBI Accession NP_005336
UniProt P08107
Indications d'application
  • WB (1:1000)
  • IHC (1:100)
  • ICC/IF (1:100)
  • IP (1:100)
  • optimal dilutions for assays should be determined by the user.
Commentaires

A 1:1000 dilution of SPC-103 was sufficient for detection of HSP70 in 20 μg of HeLa cell lysate by ECL immunoblot analysis.

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide
Stock -20 °C
Images (Fournisseur)
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Heat Shock Protein 70 (HSP70) (full length) antibody (Atto 594) (ABIN2486666) Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Hsp70 Polyclonal An...
Immunohistochemistry (IHC) image for anti-Heat Shock Protein 70 (HSP70) (full length) antibody (Atto 594) (ABIN2486666) Immunohistochemistry analysis using Rabbit Anti-HSP70 Polyclonal Antibody . Tissue: c...
Western Blotting (WB) image for anti-Heat Shock Protein 70 (HSP70) (full length) antibody (Atto 594) (ABIN2486666) Western blot analysis of Human, Rat brain cell lysates showing detection of HSP70 pro...
Immunohistochemistry (IHC) image for anti-Heat Shock Protein 70 (HSP70) (full length) antibody (Atto 594) (ABIN2486666) Immunohistochemistry analysis using Rabbit Anti-HSP70 Polyclonal Antibody . Tissue: b...
Western Blotting (WB) image for anti-Heat Shock Protein 70 (HSP70) (full length) antibody (Atto 594) (ABIN2486666) Western blot analysis of Mouse Pam212 cells showing detection of HSP70 protein using ...
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Heat Shock Protein 70 (HSP70) (full length) antibody (Atto 594) (ABIN2486666) Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Hsp70 Polyclonal An...
Immunohistochemistry (IHC) image for anti-Heat Shock Protein 70 (HSP70) (full length) antibody (Atto 594) (ABIN2486666) Immunohistochemistry analysis using Rabbit Anti-HSP70 Polyclonal Antibody . Tissue: I...
Background publications Hung, Skepper, Burton: "In vitro ischemia-reperfusion injury in term human placenta as a model for oxidative stress in pathological pregnancies." dans: The American journal of pathology, Vol. 159, Issue 3, pp. 1031-43, 2001 (PubMed).

Fink: "Chaperone-mediated protein folding." dans: Physiological reviews, Vol. 79, Issue 2, pp. 425-49, 1999 (PubMed).

Boorstein, Ziegelhoffer, Craig: "Molecular evolution of the HSP70 multigene family." dans: Journal of molecular evolution, Vol. 38, Issue 1, pp. 1-17, 1994 (PubMed).

DeLuca-Flaherty, McKay, Parham, Hill: "Uncoating protein (hsc70) binds a conformationally labile domain of clathrin light chain LCa to stimulate ATP hydrolysis." dans: Cell, Vol. 62, Issue 5, pp. 875-87, 1990 (PubMed).

Rothman: "Polypeptide chain binding proteins: catalysts of protein folding and related processes in cells." dans: Cell, Vol. 59, Issue 4, pp. 591-601, 1990 (PubMed).

Welch, Suhan: "Cellular and biochemical events in mammalian cells during and after recovery from physiological stress." dans: The Journal of cell biology, Vol. 103, Issue 5, pp. 2035-52, 1987 (PubMed).

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