AIF anticorps (AA 183-195)

N° du produit ABIN2613375

L’anticorps anti-AIF Polyclonal Chèvre est utilisé pour la détection de AIF dans des échantillons de Humain et Souris. Il a été validé pour WB, IHC, ELISA et IF.

Aperçu rapide pour AIF anticorps (AA 183-195) (ABIN2613375)

Antigène: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Reactivité: Humain, Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp AIF est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Classe de qualité: Verified

Détail du produit anti-AIF anticorps

Épitope: AA 183-195

Fonction: AIFM1 (aa183-195)

Séquence: DDPNVTKTLR FKQ

Specificité: This antibody is expected to recognize isoform 1 (NP_004199.1), isoform 2 (NP_665811.1) and isoform 5 (NP_001124319).

Réactivité croisée: Boeuf (Vache), Humain

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-DDPNVTKTLRFKQ, from the internal region of the protein sequence

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 5-7 μg/mL.

Western Blot: Approx 70 kDa band observed in lysates of cell line Jurkat (calculated MW of 66.9 kDa according to NP_004199.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:128000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the Mitochondria of HeLa and NIH3T3 cells. Recommended concentration: 5-10μg/ml.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur AIF

Antigène: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Autre désignation: AIFM1

Sujet: AIFM1, apoptosis-inducing factor, mitochondrion-associated, 1, AIF, CMTX4, COWCK, COXPD6, PDCD8, apoptosis-inducing factor 1, mitochondrial, programmed cell death 8 (apoptosis-inducing factor), striatal apoptosis-inducing factor

ID gène: 9131

NCBI Accession: NP_004199, NP_665811, NP_001124319

Pathways: Apoptose, Positive Regulation of Endopeptidase Activity, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, L'effet Warburg