Crk anticorps

N° du produit ABIN265373

L’anticorps Lapin Polyclonal anti-Crk a été validé pour WB, IF, IHC (p) et EIA. Il convient pour détecter Crk dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour Crk anticorps (ABIN265373)

Antigène: Crk (CRK) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Crk est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-Crk anticorps

Specificité: This antibody detects endogenous levels of CRK protein (region surrounding aa215).

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen

Pureté: > 95 % pure by SDS-PAGE

Information d'application

Indications d'application: ELISA: 1: 5000approx. 1: 10000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. IF: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 15 mM Sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur Crk

Antigène: Crk (CRK) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK))

Autre désignation: CRK

Sujet: Crk I and Crk II are encoded by the same gene, which undergoes alternative splicing to yield these two proteins, but differ in their biological activities. Crk II has less transforming activity than Crk I, although both Crk I and Crk II bind to many tyrosine-phosphorylated proteins that bind to GRB2. In addition, Crk II becomes rapidly tyrosine-phosphorylated in response to stimulation with insulin-like growth factor I (IGF-I) and might be involved in the IGF-I receptor signalling pathway.Synonyms: Adapter molecule crk, Proto-oncogene C-crk, p38

Poids moléculaire: approx. 42 kDa

ID gène: 1398

NCBI Accession: NP_005197

UniProt: P46108

Pathways: Neurotrophin Signaling Pathway, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor