POLB anticorps

N° du produit ABIN265385

L’anticorps anti-POLB Polyclonal Lapin est utilisé pour la détection de POLB dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB et IHC (p).

Aperçu rapide pour POLB anticorps (ABIN265385)

Antigène: POLB (Polymerase (DNA Directed), beta (POLB))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp POLB est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-POLB anticorps

Specificité: This antibody detects endogenous levels of DNA polymerase beta / DPOLB protein (region surrounding Gln324).

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen

Information d'application

Indications d'application: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur POLB

Antigène: POLB (Polymerase (DNA Directed), beta (POLB))

Autre désignation: DNA Polymerase beta

Sujet: DNA replication, recombination and repair, all of which are necessary for genomic stability, require the presence of exonucleases. In DNA replication, these enzymes are involved in the processing of Okazaki fragments, whereas in DNA repair they function to excise damaged DNA fragments and correct recombinational mismatches. These exonucleases include the family of DNA polymerases. DNA pol α, β, δ and ε are involved in DNA replication and repair. DNA pol δ and DNA pol ε are multisubunit enzymes, with DNA pol δ consisting of two subunits-p125, which interacts with the sliding DNA clamp protein PCNA, and p50. The nuclear-encoded DNA pol γ is the only DNA polymerase required for the replication of the mitochondrial DNA. DNA pol ζ is ubiquitously expressed in various tissues and mediates the cellular mechanism of damage-induced mutagenesis. DNA pol θ is a DNA polymerase-helicase that binds ATP and is involved in the repair of interstrand crosslinks.Synonyms: POLB

Poids moléculaire: approx. 38 kDa

ID gène: 5423

NCBI Accession: NP_002681

UniProt: P06746

Pathways: Réparation de l'ADN