EIF2C3 anticorps (AA 1-175)

N° du produit ABIN2668249

Il existe 2+ publications pour ce produit. L’anticorps anti-EIF2C3 Monoclonal Souris est utilisé pour la détection de EIF2C3 dans des échantillons de Humain. Il a été validé pour IF, ICC, IP et ChIP.

Aperçu rapide pour EIF2C3 anticorps (AA 1-175) (ABIN2668249)

Antigène: EIF2C3 (Eukaryotic Translation Initiation Factor 2C3 (EIF2C3))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp EIF2C3 est non-conjugé

Application: Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)

Clone: 4B1-F6

Détail du produit anti-EIF2C3 anticorps

Épitope: AA 1-175

Fonction: Ago3 antibody (mAb) (Clone 4B1-F6)

Purification: Protein G Chromatography

Immunogène: This Ago3 (Argonaute 3) antibody was raised against a recombinant protein corresponding to amino acids 1-175 of human Ago3.

Isotype: IgG2a

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 1 μg/μL

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Références pour anticorps anti-EIF2C3 (ABIN2668249)

Shang, Zhang, Xu, Xi, Zhang, Wang, Wu: "Ribozyme-enhanced single-stranded Ago2-processed interfering RNA triggers efficient gene silencing with fewer off-target effects." dans: Nature communications, Vol. 6, pp. 8430, (2015) (PubMed).

Azuma-Mukai, Oguri, Mituyama, Qian, Asai, Siomi, Siomi: "Characterization of endogenous human Argonautes and their miRNA partners in RNA silencing." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 23, pp. 7964-9, (2008) (PubMed).

Détails sur EIF2C3

Antigène: EIF2C3 (Eukaryotic Translation Initiation Factor 2C3 (EIF2C3))

Autre désignation: Ago3

Sujet: Argonaute 3 (Ago3, aka EIF2C3) is a member of the Argonaute family of proteins involved in RNAi mediated gene silencing through siRNA and miRNA effectors. The Argonaute proteins are part of RISC, the RNAi Induced Silencing Complex. Ago3, however, lacks RNase activity and does not appear to cleave target mRNA molecules. Ago3 has both a PAZ and a PIWI domain, indicative of the Argonaute family of proteins.

Poids moléculaire: 110 kDa

ID gène: 192669

NCBI Accession: NP_079128

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway