Retinoblastoma Binding Protein 8 anticorps (AA 620-897)

N° du produit ABIN2668263

Cet anticorps Souris Monoclonal détecte spécifiquement Retinoblastoma Binding Protein 8 dans WB, IP, IF, ICC et ChIP. Il présente une réactivité avec des échantillons de Humain. Il a été cité dans 6+ publications.

Aperçu rapide pour Retinoblastoma Binding Protein 8 anticorps (AA 620-897) (ABIN2668263)

Antigène: Retinoblastoma Binding Protein 8 (RBBP8)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Retinoblastoma Binding Protein 8 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP)

Clone: 14-1

Détail du produit anti-Retinoblastoma Binding Protein 8 anticorps

Épitope: AA 620-897

Fonction: CtIP antibody (mAb) (Clone 14-1)

Purification: Protein A Chromatography

Immunogène: This CtIP antibody was raised against a recombinant protein corresponding to amino acid residues 620-897 of human CtIP.

Isotype: IgG1

Information d'application

Indications d'application: WB: 1 - 2 μg/mL

Restrictions: For Research Use only

Stockage

Concentration: 1 μg/μL

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Références pour anticorps anti-Retinoblastoma Binding Protein 8 (ABIN2668263)

Onyango, Howard, Neherin, Yanez, Stark: "Tetratricopeptide repeat factor XAB2 mediates the end resection step of homologous recombination." dans: Nucleic acids research, Vol. 44, Issue 12, pp. 5702-16, (2016) (PubMed).

Bakr, Köcher, Volquardsen, Petersen, Borgmann, Dikomey, Rothkamm, Mansour: "Impaired 53BP1/RIF1 DSB mediated end-protection stimulates CtIP-dependent end resection and switches the repair to PARP1-dependent end joining in G1." dans: Oncotarget, Vol. 7, Issue 36, pp. 57679-57693, (2016) (PubMed).

Zhou, Caron, Legube, Paull: "Quantitation of DNA double-strand break resection intermediates in human cells." dans: Nucleic acids research, Vol. 42, Issue 3, pp. e19, (2014) (PubMed).

Makharashvili, Tubbs, Yang, Wang, Barton, Zhou, Deshpande, Lee, Lobrich, Sleckman, Wu, Paull: "Catalytic and noncatalytic roles of the CtIP endonuclease in double-strand break end resection." dans: Molecular cell, Vol. 54, Issue 6, pp. 1022-33, (2014) (PubMed).

Zhang, Jasin: "An essential role for CtIP in chromosomal translocation formation through an alternative end-joining pathway." dans: Nature structural & molecular biology, Vol. 18, Issue 1, pp. 80-4, (2011) (PubMed).

Yu, Fu, Lai, Baer, Chen: "BRCA1 ubiquitinates its phosphorylation-dependent binding partner CtIP." dans: Genes & development, Vol. 20, Issue 13, pp. 1721-6, (2006) (PubMed).

Détails sur Retinoblastoma Binding Protein 8

Antigène: Retinoblastoma Binding Protein 8 (RBBP8)

Autre désignation: CtIP

Sujet: CtIP (CTBP interacting protein, RBBP8, RIM, COM1) is a transcriptional regulator and tumor suppressor first identified through its interaction with CTBP (C-Terminal Binding Protein). CtIP binds to the Rb protein and serves as a transcriptional repressor, through recruitment of CtBP and also by interaction with general transcription factors TFIID and TFIIB. CtIP also interacts with Rb-related protein p130 and Ikaros, a master regulator in lymphocyte development. CtIP is phosphorylated upon DNA damage (likely by ATM and/or ATR), interacts with BRCA1 and plays a critical role in facilitating DNA damage repair. Mutations that abolish the interaction between CtIP and BRCA1 result in loss of cell cycle control leading to the development of cancer.

Poids moléculaire: 120 kDa

ID gène: 5932

NCBI Accession: NP_002885

Pathways: Cycle Cellulaire, Réparation de l'ADN