POLR2B anticorps (N-Term)

N° du produit ABIN2668294

L’anticorps anti-POLR2B Polyclonal Lapin est utilisé pour la détection de POLR2B dans des échantillons de Humain. Il a été validé pour WB et IP.

Aperçu rapide pour POLR2B anticorps (N-Term) (ABIN2668294)

Antigène: POLR2B (Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp POLR2B est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP)

Détail du produit anti-POLR2B anticorps

Épitope: N-Term

Fonction: POLR2B antibody (pAb)

Purification: Affinity Purified

Immunogène: This antibody was raised against a peptide within the N-terminal region of human POLR2B.

Isotype: IgG

Information d'application

Indications d'application: IP: 5 - 10 µL per IP WB*: 1:500 - 1:1,000 dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Détails sur POLR2B

Antigène: POLR2B (Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B))

Autre désignation: POLR2B

Sujet: POLR2B (polymerase (RNA) II (DNA directed) polypeptide B, 140 kDa) DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB2 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template (By similarity).

Poids moléculaire: 135 kDa

ID gène: 5431

NCBI Accession: NP_000929

Pathways: Regulatory RNA Pathways, Réparation de l'ADN