POLR2B anticorps (AA 805-854)

N° du produit ABIN6736747

Détail du produit anti-POLR2B anticorps

Antigène: POLR2B (Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B))

Épitope: AA 805-854

Reactivité: Humain, Souris, Rat, Chien, Boeuf (Vache), Lapin, Cobaye, Cheval, Porc, Xenopus laevis, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp POLR2B est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human POLR2B / RPB2

Homologie: Percent identity by BLAST analysis: Human, Rat, Dog, Bovine, Xenopus (100%) Chicken, Zebrafish (92%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide located between aa805-854 of human POLR2B (P30876, NP_000929). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Platypus, Xenopus (100%), Zebra finch, Chicken, Salmon, Stickleback, Zebrafish (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur POLR2B

Antigène: POLR2B (Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B))

Autre désignation: POLR2B / RPB2 (POLR2B Produits)

Synonymes: anticorps Pol2rb, anticorps Rpb140, anticorps Rpb2, anticorps POL2RB, anticorps RPB2, anticorps hRPB140, anticorps hsRPB2, anticorps RNA polymerase II subunit B, anticorps polymerase (RNA) II (DNA directed) polypeptide B, 140kDa, anticorps DNA-directed RNA polymerase II subunit RPB2, anticorps polymerase (RNA) II (DNA directed) polypeptide B, anticorps polymerase (RNA) II (DNA directed) polypeptide B, 140kDa L homeolog, anticorps POLR2B, anticorps polr2b, anticorps LOC100640211, anticorps Polr2b, anticorps polr2b.L, anticorps rpb-2

Sujet: Name/Gene ID: POLR2B

Synonyms: POLR2B, HsRPB2, POL2RB, RNA polymerase II subunit B2, RNA polymerase II subunit 2, RPB2, HRPB140

ID gène: 5431

NCBI Accession: NP_000929

UniProt: P30876

Pathways: Regulatory RNA Pathways, Réparation de l'ADN