Lamin A/C anticorps (C-Term, Mature)

N° du produit ABIN2668842

Cet anticorps Souris Monoclonal détecte spécifiquement Lamin A/C dans WB, IF, ICC et IP. Il présente une réactivité avec des échantillons de Humain.Il a été cité dans 1 publication.

Aperçu rapide pour Lamin A/C anticorps (C-Term, Mature) (ABIN2668842)

Antigène: Lamin A/C (LMNA)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Lamin A/C est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Clone: 4A4

Détail du produit anti-Lamin A/C anticorps

Épitope: C-Term, Mature

Fonction: Lamin A, mature antibody (mAb) (Clone 4A4)

Purification: Protein G Chromatography

Immunogène: This Lamin A, mature antibody was raised against a peptide within the C-terminus of mature human Lamin A.

Isotype: IgG1

Information d'application

Indications d'application: WB*: 0.05 - 2 μg/mL *Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Restrictions: For Research Use only

Stockage

Concentration: 1 μg/μL

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Références pour anticorps anti-Lamin A/C (ABIN2668842)

Roblek, Schüchner, Huber, Ollram, Vlcek-Vesely, Foisner, Wehnert, Ogris: "Monoclonal antibodies specific for disease-associated point-mutants: lamin A/C R453W and R482W." dans: PLoS ONE, Vol. 5, Issue 5, pp. e10604, (2010) (PubMed).

Détails sur Lamin A/C

Antigène: Lamin A/C (LMNA)

Autre désignation: Lamin A

Sujet: Nuclear lamins are intermediate filament proteins that are the major structural component of the nuclear lamina on the inner surface of the nuclear envelope. Lamins A and Lamins C are splice variants of the Lamin A gene. Lamin A/C (CDCD1, LMN1, EMD2) expression is a hallmark of embryonic stem cell differentiation. In addition to adding structural integrity to the nucleus, lamins contribute to the makeup of the nuclear matrix. Lamins also help organize interphase chromatin through interactions with several chromatin proteins, including histones and Lap2, such that alteration in lamin organization results in disruption of DNA replication, transcription and RNA processing.

Poids moléculaire: 74 kDa

ID gène: 4000

NCBI Accession: NP_733821

Pathways: Apoptose, Caspase Cascade in Apoptosis, ER-Nucleus Signaling, Protein targeting to Nucleus