CD11b anticorps

N° du produit ABIN2688906

L’anticorps Rat Monoclonal anti-CD11b a été validé pour FACS, WB, IP, IHC (fro), IHC (p) et BR. Il convient pour détecter CD11b dans des échantillons de Humain et Souris. Il y a 8+ publications disponibles.

Aperçu rapide pour CD11b anticorps (ABIN2688906)

Antigène: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reactivité: Humain, Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp CD11b est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Blocking Reagent (BR)

Clone: M1-70

Détail du produit anti-CD11b anticorps

Marque: BD Pharmingen™

Attributs du produit: The M1/70 antibody reacts with the 170 kDa α[M] chain of Mac-1 (CD11b/CD18, α[M]β2 integrin), also known as complement receptor 3 (CR3), which mediates adhesion to C3bi and ICAM-1 (CD54). Mac-1 is expressed at varying levels on granulocytes, macrophages, myeloid-derived dendritic cells, natural killer cells, microglia, and B-1 cells. Mac-1 expression is rapidly up-regulated on neutrophils after activation, in the same time period that CD62L (L-selectin) is shed from the cell surface. M1/70 antibody reportedly blocks cell adherence and C3bi binding, but it does not block cell-mediated lysis. Cross-reaction of mAb M1/70 with CD11b on human monocytes, polymorphonuclear leukocytes, and NK cells has been reported. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Expression of CD11b on bone-marrow myeloid cells. BALB/c bone-marrow leukocytes were stained with either purified Rat IgG2b, κ isotype control A95-1 (Cat. No. 553986, left panel) or purified M1/70 monoclonal antibodies (right panel), followed by FITC-conjugated goat anti-rat Ig (Cat. No. 554016, both panels). Please note that the population of cells having the lowest SSC (erythroid and lymphoid cells) show little expression of CD11b, while cells with moderate-to-high SSC (myeloid cells) are almost uniformly CD11b positive (right panel). Flow cytometry was performed on a BD FACScan™ flow cytometry system.

BD Pharmingen™ Purified Rat Anti-Mouse CD11b - Purified - Clone M1/70 - Isotype Rat IgG2b, κ - Reactivity Ms, Hu - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: C57BL/10 splenic T cells and concanavalin A-activated C57BL/10 splenocytes

Isotype: IgG2b kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-CD11b (ABIN2688906)

Lagasse, Weissman: "Flow cytometric identification of murine neutrophils and monocytes." dans: Journal of immunological methods, Vol. 197, Issue 1-2, pp. 139-50, (1996) (PubMed).

Lub, van Kooyk, Figdor: "Competition between lymphocyte function-associated antigen 1 (CD11a/CD18) and Mac-1 (CD11b/CD18) for binding to intercellular adhesion molecule-1 (CD54)." dans: Journal of leukocyte biology, Vol. 59, Issue 5, pp. 648-55, (1996) (PubMed).

Kishimoto, Jutila, Berg, Butcher: "Neutrophil Mac-1 and MEL-14 adhesion proteins inversely regulated by chemotactic factors." dans: Science (New York, N.Y.), Vol. 245, Issue 4923, pp. 1238-41, (1989) (PubMed).

Beller, Springer, Schreiber: "Anti-Mac-1 selectively inhibits the mouse and human type three complement receptor." dans: The Journal of experimental medicine, Vol. 156, Issue 4, pp. 1000-9, (1983) (PubMed).

Flotte, Springer, Thorbecke: "Dendritic cell and macrophage staining by monoclonal antibodies in tissue sections and epidermal sheets." dans: The American journal of pathology, Vol. 111, Issue 1, pp. 112-24, (1983) (PubMed).

Springer, Davignon, Ho, Kürzinger, Martz, Sanchez-Madrid: "LFA-1 and Lyt-2,3, molecules associated with T lymphocyte-mediated killing; and Mac-1, an LFA-1 homologue associated with complement receptor function." dans: Immunological reviews, Vol. 68, pp. 171-95, (1983) (PubMed).

Springer, Galfré, Secher, Milstein: "Mac-1: a macrophage differentiation antigen identified by monoclonal antibody." dans: European journal of immunology, Vol. 9, Issue 4, pp. 301-6, (1979) (PubMed).

Springer, Galfrè, Secher, Milstein: "Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens." dans: European journal of immunology, Vol. 8, Issue 8, pp. 539-51, (1978) (PubMed).

Détails sur CD11b

Antigène: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Autre désignation: CD11b

Sujet: Synonyms: Integrin α[M] chain, Mac-1 α chain, CR3

Pathways: Apoptose, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation