FASL anticorps

N° du produit ABIN2689020

L’anticorps Souris Monoclonal anti-FASL a été validé pour WB, FACS, ELISA, IP et Neut. Il convient pour détecter FASL dans des échantillons de Humain. Il y a 3+ publications disponibles.

Aperçu rapide pour FASL anticorps (ABIN2689020)

Antigène: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp FASL est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunoprecipitation (IP), Neutralization (Neut)

Clone: NOK

Détail du produit anti-FASL anticorps

Marque: BD Pharmingen™

Attributs du produit: Fas (APO-1, CD95) is a 45 kDa cell surface protein that mediates apoptosis when cross-linked with agonistic anti-Fas antibodies or Fas ligand (FasL). Fas belongs to the TNF (tumor necrosis factor)/NGF (nerve growth factor) receptor family, and is expressed in various tissue and cells including the thymus, liver, ovary, and lung. FasL is a 40 kDa TNF family membrane protein that induces apoptosis by binding to Fas, its cell-surface receptor. FasL is expressed on activated T and NK cells. Both Fas and FasL are thought to play an important role in the apoptotic processes that take place during T cell development. NOK-2 recognizes human FasL. It recognizes both the membrane bound (FasL) and soluble (sFasL) forms. L5178Y mouse T lymphoma cells expressing recombinant human FasL were used as immunogen. FasL and sFasL migrate at reduced molecular weights of 40 and 26 kDa, respectively. However, the molecular weights observed in a particular sample may vary according to FasL and sFasL glycosylation and breakdown patterns as described in Tanaka et al. Immunoprecipitation of Fas Ligand (FasL). T lymphoma L5187Y cells were transfected with human FasL cDNA and treated or not treated with metalloprotease inhibitor KB8301. KB8301 blocks FasL cleavage resulting in high levels of cell surface FasL. FasL is detected in S-labeled cells treated with inhibitor KB8301 (lane 1), but not in untreated cells (lane 3). Isotype (negative) controls are shown in lanes 2 and 4. Purified NOK-2 (Cat. No. 556376) or mouse IgG2a (Cat. No. 553454) was used for immunoprecipitations.

BD Pharmingen™ Purified Mouse Anti-Human CD178 - Purified - Clone NOK-2 - Isotype Mouse IgG2a - Reactivity Hu - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: L51788Y Mouse T Lymphoma Cells Expressing Recombinant Human FasL

Isotype: IgG2a

Information d'application

Indications d'application: Flow Cytometry : Another clone, NOK-1, (556372, 556373) are recommended for flow cytometry. NOK-2 appears weaker for flow cytometry than NOK-1. Western Blot: Neither NOK-1 nor NOK-2 are suggested for western blot analysis. Another human FasL clone, G247-4 is suggested for the western blot analysis.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-FASL (ABIN2689020)

Kayagaki, Kawasaki, Ebata, Ohmoto, Ikeda, Inoue, Yoshino, Okumura, Yagita: "Metalloproteinase-mediated release of human Fas ligand." dans: The Journal of experimental medicine, Vol. 182, Issue 6, pp. 1777-83, (1996) (PubMed).

Tanaka, Suda, Takahashi, Nagata: "Expression of the functional soluble form of human fas ligand in activated lymphocytes." dans: The EMBO journal, Vol. 14, Issue 6, pp. 1129-35, (1995) (PubMed).

Takahashi, Tanaka, Brannan, Jenkins, Copeland, Suda, Nagata: "Generalized lymphoproliferative disease in mice, caused by a point mutation in the Fas ligand." dans: Cell, Vol. 76, Issue 6, pp. 969-76, (1994) (PubMed).

Détails sur FASL

Antigène: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Autre désignation: CD178

Sujet: Synonyms: Fas Ligand, CD95 Ligand

Poids moléculaire: 40, 26 kDa

Pathways: Apoptose, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity