TRAIL anticorps

N° du produit ABIN2689128

Détail du produit anti-TRAIL anticorps

Antigène: TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp TRAIL est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS)

Marque: BD Pharmingen™

Attributs du produit: The N2B2 antibody reacts with mouse TRAIL (TNF-Related Apoptosis-Inducing Ligand, also known as APO-2 ligand or Ly-81), a 40- kDa type-II transmembrane protein of the TNF family. This antibody recognizes mouse Trail-transfected 2PK-3 cells, but not the parental cell line. TRAIL selectively induces apoptosis in various transformed cells, but not in normal cells. Five receptors have been identified for human TRAIL, whereas only one receptor has been described for the mouse counterpart thus far. The pattern of expression of mouse TRAIL is controversial. TRAIL has been detected, with a rabbit polyclonal antibody, on the surface of activated CD4+ and CD8+ T lymphocytes, as well as on CD45R/B220+ lymphocytes, and transformed T- and B-cell lines. Similar results were obtained by western blot and mRNA analyses. However, these results could not be reproduced with the N2B2 mAb. TRAIL expression, detected with the N2B2 mAb, is induced on CD3- NK1.1+ cells after stimulation with IL-2 or IL-15. However, neither freshly isolated nor activated T cells showed TRAIL expression on the cell surface. Furthermore, TRAIL mediates NK-cell cytotoxicity, which is blocked by the N2B2 mAb. TRAIL has been renamed as CD253 recently.

BD Pharmingen™ Purified Rat Anti-Mouse CD253 - Purified - Clone N2B2 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Mouse Trail-transfected 2PK-3 cells

Clone: N2B2

Isotype: IgG2a kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-TRAIL (ABIN2689128)

Degli-Esposti: "To die or not to die--the quest of the TRAIL receptors." dans: Journal of leukocyte biology, Vol. 65, Issue 5, pp. 535-42, (1999) (PubMed).

Kayagaki, Yamaguchi, Nakayama, Takeda, Akiba, Tsutsui, Okamura, Nakanishi, Okumura, Yagita: "Expression and function of TNF-related apoptosis-inducing ligand on murine activated NK cells." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 4, pp. 1906-13, (1999) (PubMed).

Mariani, Krammer: "Surface expression of TRAIL/Apo-2 ligand in activated mouse T and B cells." dans: European journal of immunology, Vol. 28, Issue 5, pp. 1492-8, (1998) (PubMed).

Mariani, Krammer: "Differential regulation of TRAIL and CD95 ligand in transformed cells of the T and B lymphocyte lineage." dans: European journal of immunology, Vol. 28, Issue 3, pp. 973-82, (1998) (PubMed).

Détails sur TRAIL

Antigène: TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))

Autre désignation: CD253 (TNFSF10 Produits)

Synonymes: anticorps APO2L, anticorps Apo-2L, anticorps CD253, anticorps TL2, anticorps TRAIL, anticorps A330042I21Rik, anticorps AI448571, anticorps APO-2L, anticorps Ly81, anticorps Trail, anticorps tl2, anticorps apo2l, anticorps cd253, anticorps trail, anticorps apo-2l, anticorps trail1, anticorps xtrail1, anticorps tnfsf10l2, anticorps zgc:92320, anticorps TNLG6A, anticorps TNF superfamily member 10, anticorps tumor necrosis factor (ligand) superfamily, member 10, anticorps tumor necrosis factor superfamily member 10, anticorps TNF superfamily member 10 S homeolog, anticorps TNFSF10, anticorps Tnfsf10, anticorps tnfsf10.S, anticorps tnfsf10

Sujet: Synonyms: TRAIL, APO-2L, Ly-81, TL2

Pathways: Apoptose, Positive Regulation of Endopeptidase Activity