CD28 anticorps

N° du produit ABIN2689144

Détail du produit anti-CD28 anticorps

Antigène: CD28

Reactivité: Souris

Hôte: Golden Syrian Hamster

Clonalité: Monoclonal

Conjugué: Cet anticorp CD28 est non-conjugé

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Blocking Reagent (BR), Cytotoxicity Test (CyTox)

Marque: BD Pharmingen™

Attributs du produit: The 37.51 antibody reacts with CD28, which is expressed on most thymocytes, at low density on nearly all CD4+ and CD8+ peripheral T cells, and at even lower density on NK cells. The expression of CD28, in splenocytes and thymocytes, has been reported to increase after activation. CD28 transcripts are found in mast cells, and cell-surface expression of CD28 is induced upon maturation or activation of mast cells. It has been reported that CD28 is not expressed on some populations of intraepithelial T lymphocytes. CD28 is a costimulatory receptor, its ligands include CD80 (B7-1) and CD86 (B7-2). The 37.51 mAb augments proliferation and cytokine production by activated T and NK cells and can provide a costimulatory signal for CTL induction. There is considerable evidence that CD28 is a costimulatory receptor involved in many, but not all, T cell-dependent immune responses. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Two-color analysis of CD28 expression on splenic T lymphocytes. BALB/c splenocytes were preincubated with purified anti-mouse CD16/CD32 mAb [Mouse BD Fc Block™] (Cat. No. 553141), then stained simultaneously with FITC-conjugated anti-mouse CD4 mAb RM4-5 (Cat. No. 553046) and FITC-conjugated anti-mouse CD8a mAb 53-6.7 (Cat. No. 553030) in addition to staining with purified anti-mouse CD28 clone 37.51 (right panel). Staining of CD28-positive cells was detected with biotinylated anti-Syrian hamster IgG2 mAb G192-3 (Cat. No. 554029), followed by streptavidin-PE (Cat. No. 554061). Flow cytometry was performed on a BD FACScan™ flow cytometry system.

BD Pharmingen™ Purified Hamster Anti-Mouse CD28 - Purified - Clone 37.51 - Isotype Syrian Hamster IgG2, λ1 - Reactivity Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Mouse EL-4 (T-cell lymphoma) Cells

Clone: 37-51

Isotype: IgG2 lambda

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-CD28 (ABIN2689144)

Cibotti, Punt, Dash, Sharrow, Singer: "Surface molecules that drive T cell development in vitro in the absence of thymic epithelium and in the absence of lineage-specific signals." dans: Immunity, Vol. 6, Issue 3, pp. 245-55, (1997) (PubMed).

Marietta, Weis, Weis: "CD28 expression by mouse mast cells is modulated by lipopolysaccharide and outer surface protein A lipoprotein from Borrelia burgdorferi." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 6, pp. 2840-8, (1997) (PubMed).

Masten, Yates, Pollard Koga, Lipscomb: "Characterization of accessory molecules in murine lung dendritic cell function: roles for CD80, CD86, CD54, and CD40L." dans: American journal of respiratory cell and molecular biology, Vol. 16, Issue 3, pp. 335-42, (1997) (PubMed).

Rakasz, Hagen, Sandor, Lynch: "Gamma delta T cells of the murine vagina: T cell response in vivo in the absence of the expression of CD2 and CD28 molecules." dans: International immunology, Vol. 9, Issue 1, pp. 161-7, (1997) (PubMed).

Nishio, Spielman, Lee, Nelson, Podack: "CD80 (B7.1) and CD54 (intracellular adhesion molecule-1) induce target cell susceptibility to promiscuous cytotoxic T cell lysis." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 10, pp. 4347-53, (1996) (PubMed).

Bluestone: "New perspectives of CD28-B7-mediated T cell costimulation." dans: Immunity, Vol. 2, Issue 6, pp. 555-9, (1995) (PubMed).

Gelfanov, Lai, Gelfanova, Dong, Su, Liao: "Differential requirement of CD28 costimulation for activation of murine CD8+ intestinal intraepithelial lymphocyte subsets and lymph node cells." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 1, pp. 76-82, (1995) (PubMed).

June, Bluestone, Nadler, Thompson: "The B7 and CD28 receptor families." dans: Immunology today, Vol. 15, Issue 7, pp. 321-31, (1994) (PubMed).

Nandi, Gross, Allison: "CD28-mediated costimulation is necessary for optimal proliferation of murine NK cells." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 7, pp. 3361-9, (1994) (PubMed).

Harding, Allison: "CD28-B7 interactions allow the induction of CD8+ cytotoxic T lymphocytes in the absence of exogenous help." dans: The Journal of experimental medicine, Vol. 177, Issue 6, pp. 1791-6, (1993) (PubMed).

Shahinian, Pfeffer, Lee, Kündig, Kishihara, Wakeham, Kawai, Ohashi, Thompson, Mak: "Differential T cell costimulatory requirements in CD28-deficient mice." dans: Science (New York, N.Y.), Vol. 261, Issue 5121, pp. 609-12, (1993) (PubMed).

Gross, Callas, Allison: "Identification and distribution of the costimulatory receptor CD28 in the mouse." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 2, pp. 380-8, (1992) (PubMed).

Harding, McArthur, Gross, Raulet, Allison: "CD28-mediated signalling co-stimulates murine T cells and prevents induction of anergy in T-cell clones." dans: Nature, Vol. 356, Issue 6370, pp. 607-9, (1992) (PubMed).

Détails sur CD28

Antigène: CD28

Autre désignation: CD28 (CD28 Produits)

Synonymes: anticorps Tp44, anticorps CD28RNA, anticorps cd28, anticorps CD28 molecule, anticorps CD28 antigen, anticorps Cd28 molecule, anticorps CD28 molecule L homeolog, anticorps CD28, anticorps Cd28, anticorps cd28.L

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response