CD3 epsilon anticorps

N° du produit ABIN2689213

Détail du produit anti-CD3 epsilon anticorps

Antigène: CD3 epsilon (CD3E)

Reactivité: Souris

Hôte: Golden Syrian Hamster

Clonalité: Monoclonal

Conjugué: Cet anticorp CD3 epsilon est non-conjugé

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Marque: BD Pharmingen™

Attributs du produit: This antibody has been reported to react with the 25- kDa ε chain of the T-cell receptor-associated CD3 complex expressed on thymocytes, mature T lymphocytes, and NK-T cells on many mouse stains. Plate-bound and soluble hamster anti-mouse CD3e (clone 500A2) antibody has been reported to activate unprimed T cells in vitro. Activation of a mouse T-cell clone by soluble hamster anti-mouse CD3e (clone 500A2) can be blocked by Fab fragments of anti-CD4 antibody GK1.5, suggesting that the hamster anti-mouse CD3e (clone 500A2) antibody may bind an epitope of CD3e close to a site at which CD4 associates with the T-cell receptor. This hamster mAb to a mouse leukocyte antigen has been reported not to cross-react with rat leukocytes. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. CD3e expression in spleen and thymus. BALB/c splenocytes were simultaneously stained with PE-conjugated anti-mouse CD4 mAb RM4-5 (Cat. No. 553048/553049, left panels), PE-conjugated anti-mouse CD8a mAb 53-6.7 (Cat. No. 553032/553033, left panels), and purified mAb 500A2 (bottom left panel), followed by FITC-conjugated anti-hamster IgG mAb G192-1 (Cat. No. 554026, left panels). BALB/c thymocytes were stained with purified 500A2 (bottom right panel) followed by FITC-conjugated G192-1 mAb (right panels). Flow cytometry was performed on a BD FACScan™ flow cytometry system.

BD Pharmingen™ Purified Hamster Anti-Mouse CD3e - Purified - Clone 500A2 - Isotype Syrian Hamster IgG2, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Mouse T-cell receptor

Clone: 500A2

Isotype: IgG2 kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-CD3 epsilon (ABIN2689213)

Ortaldo, Winkler-Pickett, Mason, Mason: "The Ly-49 family: regulation of cytotoxicity and cytokine production in murine CD3+ cells." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 3, pp. 1158-65, (1998) (PubMed).

Jacobs, Vandeputte, Tolkamp, de Vries, Borst, Berns: "CD3 components at the surface of pro-T cells can mediate pre-T cell development in vivo." dans: European journal of immunology, Vol. 24, Issue 4, pp. 934-9, (1994) (PubMed).

Kubo, Born, Kappler, Marrack, Pigeon: "Characterization of a monoclonal antibody which detects all murine alpha beta T cell receptors." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 8, pp. 2736-42, (1989) (PubMed).

Portoles, Rojo, Golby, Bonneville, Gromkowski, Greenbaum, Janeway, Murphy, Bottomly: "Monoclonal antibodies to murine CD3 epsilon define distinct epitopes, one of which may interact with CD4 during T cell activation." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 12, pp. 4169-75, (1989) (PubMed).

Havran, Poenie, Kimura, Tsien, Weiss, Allison: "Expression and function of the CD3-antigen receptor on murine CD4+8+ thymocytes." dans: Nature, Vol. 330, Issue 6144, pp. 170-3, (1987) (PubMed).

Détails sur CD3 epsilon

Antigène: CD3 epsilon (CD3E)

Autre désignation: CD3e (CD3E Produits)

Synonymes: anticorps cd3e, anticorps CD3E, anticorps t3e, anticorps tcre, anticorps AI504783, anticorps CD3, anticorps CD3epsilon, anticorps T3e, anticorps T3E, anticorps TCRE, anticorps CD3e molecule, anticorps CD3 antigen, epsilon polypeptide, anticorps cd3e, anticorps CD3E, anticorps Cd3e

Sujet: Synonyms: CD3ε chain

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway