CD3 epsilon anticorps

N° du produit ABIN2689215

Détail du produit anti-CD3 epsilon anticorps

Antigène: CD3 epsilon (CD3E)

Reactivité: Souris

Hôte: Hamster arménien

Clonalité: Monoclonal

Conjugué: Cet anticorp CD3 epsilon est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Blocking Reagent (BR), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc))

Marque: BD Pharmingen™

Attributs du produit: The 145-2C11 antibody reacts with the 25- kDa ε chain of the T-cell receptor-associated CD3 complex, which is expressed on thymocytes, mature T lymphocytes, and NK-T cells. The cytoplasmic domain of CD3e participates in the signal transduction events which activate several cellular biochemical pathways as a result of antigen recognition. Soluble 145-2C11 antibody can activate either unprimed (naive) or primed (memory/preactivated) T cells in vivo or in vitro, in the presence of Fc receptor-bearing accessory cells. In contrast, plate-bound 145-2C11 can activate T cells in the absence of accessory cells. Soluble 145-2C11 antibody has been reported to induce re-directed lysis of Fc receptor-bearing target cells by CTL clones and can also block lysis of specific target cells by antigen-specific CTL's. Under some conditions, T-cell activation by 145-2C11 antibody has been reported to result in apoptotic cell death. The 145-2C11 antibody does not cross-react with rat leukocytes and it has been reported that pre-incubation of thymus cell suspensions at 37 °C for 2-4 hours prior to staining enhances the ability of anti-CD3ε and anti-αβ TCR mAbs to detect the T-cell receptor on immature thymocytes. Immunohistochemical staining of T lymphocytes. The frozen section of normal mouse spleen was reacted with 145-2C11 mAb. The positive staining is represented by the brown labeling of cell-surface membranes of lymphocytes in the periarteriolar sheath.

BD Pharmingen™ Purified Hamster Anti-Mouse CD3e - Purified - Clone 145-2C11 - Isotype Armenian Hamster IgG1, κ - Reactivity Ms - 1 mL

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: H-2Kb specific cytotoxic T lymphocyte clone BM10-37

Clone: 145

Isotype: IgG1 kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 31.25 μg/mL

Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-CD3 epsilon (ABIN2689215)

Castro, Listman, Jacobson, Wang, Lopez, Ju, Finn, Perkins: "Fas modulation of apoptosis during negative selection of thymocytes." dans: Immunity, Vol. 5, Issue 6, pp. 617-27, (1997) (PubMed).

Nakano, Yamazaki, Miyatake, Nozaki, Kikuchi, Saito: "Specific interaction of topoisomerase II beta and the CD3 epsilon chain of the T cell receptor complex." dans: The Journal of biological chemistry, Vol. 271, Issue 11, pp. 6483-9, (1996) (PubMed).

Shinkai, Alt: "CD3 epsilon-mediated signals rescue the development of CD4+CD8+ thymocytes in RAG-2-/- mice in the absence of TCR beta chain expression." dans: International immunology, Vol. 6, Issue 7, pp. 995-1001, (1994) (PubMed).

Salvadori, Gansbacher, Pizzimenti, Zier: "Abnormal signal transduction by T cells of mice with parental tumors is not seen in mice bearing IL-2-secreting tumors." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 11, pp. 5176-82, (1994) (PubMed).

Leo, Foo, Sachs, Samelson, Bluestone: "Identification of a monoclonal antibody specific for a murine T3 polypeptide." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 5, pp. 1374-8, (1987) (PubMed).

Détails sur CD3 epsilon

Antigène: CD3 epsilon (CD3E)

Autre désignation: CD3e (CD3E Produits)

Synonymes: anticorps cd3e, anticorps CD3E, anticorps t3e, anticorps tcre, anticorps AI504783, anticorps CD3, anticorps CD3epsilon, anticorps T3e, anticorps T3E, anticorps TCRE, anticorps CD3e molecule, anticorps CD3 antigen, epsilon polypeptide, anticorps cd3e, anticorps CD3E, anticorps Cd3e

Sujet: Synonyms: CD3ε chain

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway