CD3 epsilon anticorps (Biotin)

N° du produit ABIN2689216

Détail du produit anti-CD3 epsilon anticorps (Biotin)

Antigène: CD3 epsilon (CD3E)

Reactivité: Souris

Hôte: Hamster arménien

Clonalité: Monoclonal

Conjugué: Cet anticorp CD3 epsilon est conjugé à/à la Biotin

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Marque: BD Pharmingen™

Attributs du produit: The 145-2C11 antibody reacts with the 25- kDa ε chain of the T-cell receptor-associated CD3 complex, which is expressed on thymocytes, mature T lymphocytes, and NK-T cells. The cytoplasmic domain of CD3e participates in the signal transduction events which activate several cellular biochemical pathways as a result of antigen recognition. Soluble 145-2C11 antibody can activate either unprimed (naive) or primed (memory/preactivated) T cells in vivo or in vitro, in the presence of Fc receptor-bearing accessory cells. In contrast, plate-bound 145-2C11 can activate T cells in the absence of accessory cells. Soluble 145-2C11 antibody has been reported to induce re-directed lysis of Fc receptor-bearing target cells by CTL clones and can also block lysis of specific target cells by antigen-specific CTL's. Under some conditions, T-cell activation by 145-2C11 antibody has been reported to result in apoptotic cell death. The 145-2C11 antibody does not cross-react with rat leukocytes and it has been reported that pre-incubation of thymus cell suspensions at 37 °C for 2-4 hours prior to staining enhances the ability of anti-CD3ε and anti-αβ TCR mAbs to detect the T-cell receptor on immature thymocytes. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. CD3e expression in spleen and thymus. BALB/c splenocytes were simultaneously stained with PE-conjugated anti-mouse CD4 mAb RM4-5 (Cat. No. 553048), PE-conjugated anti-mouse CD8a mAb 53-6.7 (Cat. No. 553032) and biotin-conjugated mAb 145-2C11 (bottom left panel), followed by Avidin-FITC (cat. No. 554057). BALB/c thymocytes were also stained with biotin-conjugated mAb 145-2C11 followed by Avidin-FITC (bottom right panel). Flow cytometry was performed in a BD FACScan™ flow cytometry system. Page 1 of 2553059 Rev. 18

BD Pharmingen™ Biotin Hamster Anti-Mouse CD3e - Biotin - Clone 145-2C11 - Isotype Armenian Hamster IgG1, κ - Reactivity Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: H-2Kb specific cytotoxic T lymphocyte clone BM10-37

Clone: 145

Isotype: IgG1 kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-CD3 epsilon (Biotin) (ABIN2689216)

Zheng, Han, Kelsoe: "T helper cells in murine germinal centers are antigen-specific emigrants that downregulate Thy-1." dans: The Journal of experimental medicine, Vol. 184, Issue 3, pp. 1083-91, (1997) (PubMed).

Nakano, Yamazaki, Miyatake, Nozaki, Kikuchi, Saito: "Specific interaction of topoisomerase II beta and the CD3 epsilon chain of the T cell receptor complex." dans: The Journal of biological chemistry, Vol. 271, Issue 11, pp. 6483-9, (1996) (PubMed).

Isakov, Wange, Burgess, Watts, Aebersold, Samelson: "ZAP-70 binding specificity to T cell receptor tyrosine-based activation motifs: the tandem SH2 domains of ZAP-70 bind distinct tyrosine-based activation motifs with varying affinity." dans: The Journal of experimental medicine, Vol. 181, Issue 1, pp. 375-80, (1995) (PubMed).

Shinkai, Alt: "CD3 epsilon-mediated signals rescue the development of CD4+CD8+ thymocytes in RAG-2-/- mice in the absence of TCR beta chain expression." dans: International immunology, Vol. 6, Issue 7, pp. 995-1001, (1994) (PubMed).

Kubo, Born, Kappler, Marrack, Pigeon: "Characterization of a monoclonal antibody which detects all murine alpha beta T cell receptors." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 8, pp. 2736-42, (1989) (PubMed).

Portoles, Rojo, Golby, Bonneville, Gromkowski, Greenbaum, Janeway, Murphy, Bottomly: "Monoclonal antibodies to murine CD3 epsilon define distinct epitopes, one of which may interact with CD4 during T cell activation." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 12, pp. 4169-75, (1989) (PubMed).

Leo, Foo, Sachs, Samelson, Bluestone: "Identification of a monoclonal antibody specific for a murine T3 polypeptide." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 5, pp. 1374-8, (1987) (PubMed).

Détails sur CD3 epsilon

Antigène: CD3 epsilon (CD3E)

Autre désignation: CD3e (CD3E Produits)

Synonymes: anticorps cd3e, anticorps CD3E, anticorps t3e, anticorps tcre, anticorps AI504783, anticorps CD3, anticorps CD3epsilon, anticorps T3e, anticorps T3E, anticorps TCRE, anticorps CD3e molecule, anticorps CD3 antigen, epsilon polypeptide, anticorps cd3e, anticorps CD3E, anticorps Cd3e

Sujet: Synonyms: CD3ε chain

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway