ITGA5 anticorps (Integrin, alpha 5 (Fibronectin Receptor, alpha Polypeptide)) Primary Antibody
- Cet anticorp ITGA5 est non-conjugé
- Blocking Reagent (BR), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- BD Pharmingen™
- Attributs du produit
- The 5H10-27 (MFR5) antibody reacts with the α5 chain of the integrin α5β1 fibronectin receptor (CD49e/CD29, VLA-5) on thymocytes, activated T lymphocytes, mast cells, and a variety of mouse cell lines, but not splenocytes. Soluble 5H10-27 (MFR5) antibody has been reported to inhibit VLA-5-mediated functions in vitro. In addition, immobilized mAb 5H10-27 (MFR5) has been demonstrated to costimulate the proliferative response of CD8+ T cells to plate-bound anti-CD3e antibody. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.
BD Pharmingen™ Purified Rat Anti-Mouse CD49e - Purified - Clone 5H10-27 (MFR5) - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Mouse mast cell line MC/9
- IgG2a, kappa
- Indications d'application
- Optimal working dilution should be determined by the investigator.
- For Research Use only
- 0.5 mg/mL
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 4 °C
- Stockage commentaire
- Store undiluted at 4°C.
Makwana, Werner, Acosta-Saltos, Gonitel, Pararajasingam, Pararajasingham, Ruff, Rumajogee, Cuthill, Galiano, Bohatschek, Wallace, Anderson, Mayer, Behrens, Raivich: "Peripheral facial nerve axotomy in mice causes sprouting of motor axons into perineuronal central white matter: time course and molecular characterization." dans: The Journal of comparative neurology, Vol. 518, Issue 5, pp. 699-721, 2010 (PubMed).
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Rich, Van Nood, Lee: "Role of alpha 5 beta 1 integrin in TGF-beta 1-costimulated CD8+ T cell growth and apoptosis." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 7, pp. 2916-23, 1996 (PubMed).
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Uhlenkott, Huijzer, Cardeiro, Elstad, Meadows: "Attachment, invasion, chemotaxis, and proteinase expression of B16-BL6 melanoma cells exhibiting a low metastatic phenotype after exposure to dietary restriction of tyrosine and phenylalanine." dans: Clinical & experimental metastasis, Vol. 14, Issue 2, pp. 125-37, 1996 (PubMed).
Gomez, Cano: "Expression of beta 1 integrin receptors in transformed mouse epidermal keratinocytes: upregulation of alpha 5 beta 1 in spindle carcinoma cells." dans: Molecular carcinogenesis, Vol. 12, Issue 3, pp. 153-65, 1995 (PubMed).
Halvorson, Coligan: "Enhancement of VLA integrin receptor function on thymocytes by cAMP is dependent on the maturation stage of the thymocytes." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 10, pp. 4567-74, 1995 (PubMed).
Hardy, Minguell: "Modulation of the adhesion of hemopoietic progenitor cells to the RGD site of fibronectin by interleukin 3." dans: Journal of cellular physiology, Vol. 164, Issue 2, pp. 315-23, 1995 (PubMed).
Kinashi, Springer: "Adhesion molecules in hematopoietic cells." dans: Blood cells, Vol. 20, Issue 1, pp. 25-44, 1995 (PubMed).
- Makwana, Werner, Acosta-Saltos, Gonitel, Pararajasingam, Pararajasingham, Ruff, Rumajogee, Cuthill, Galiano, Bohatschek, Wallace, Anderson, Mayer, Behrens, Raivich: "Peripheral facial nerve axotomy in mice causes sprouting of motor axons into perineuronal central white matter: time course and molecular characterization." dans: The Journal of comparative neurology, Vol. 518, Issue 5, pp. 699-721, 2010 (PubMed).