Selectin E/CD62e anticorps

N° du produit ABIN2689356

Détail du produit anti-Selectin E/CD62e anticorps

Antigène: Selectin E/CD62e (SELE) (Selectin E (SELE))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp Selectin E/CD62e est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Marque: BD Pharmingen™

Attributs du produit: The 10E9.6 antibody reacts with the 97-110 kDa cell surface glycoprotein E-selectin (CD62E), also known as endothelial-leukocyte adhesion molecule-1 (ELAM-1), which is expressed on endotoxin- or cytokine-stimulated mouse endothelial cells. A suspension of TNFα stimulated mouse brain capillary endothelioma cells, from the cell line bEnd.3, was used as the immunogen. The epitope recognized by mAb 10E9.6 has been mapped to the first and/or second complement regulatory protein repeat domains of E-selectin. The 10E9.6 antibody has been reported to block binding of a monocyte cell line to E-selectin in vitro and to block neutrophil migration in BALB/c, but not C57BL/6 mice. It has no effect on leukocyte rolling in TNFα-treated mouse venules or on in vitro adhesion of myeloid cells to E-selectin. Studies have demonstrated that Cutaneous Lymphocyte Antigen (CLA), recognized by mAb HECA-452 (Cat. no. 555946), may be a ligand for CD62E. Immunohistochemical staining for CD62E. Frozen tongue sections of a lipopolysaccharide treated C57BL/6 mouse was reacted with the 10E9.6 antibody. Endothelial cells of the blood vessels express CD62E and can be identifed by the brown staining.

BD Pharmingen™ Purified Rat Anti-Mouse CD62E - Purified - Clone 10E9.6 - Isotype Rat IgG2a, κ - Reactivity Ms - 1 mL

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Mouse brain capillary endothelioma bEnd.3 (TNFalpha-stimulated)

Clone: 10E9-6

Isotype: IgG2a kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 125 μg/mL

Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-Selectin E/CD62e (ABIN2689356)

Ramos, Kunkel, Lawrence, Jung, Vestweber, Bosse, McIntyre, Gillooly, Norton, Wolitzky, Ley: "Differential effect of E-selectin antibodies on neutrophil rolling and recruitment to inflammatory sites." dans: Blood, Vol. 89, Issue 8, pp. 3009-18, (1997) (PubMed).

Borges, Pendl, Eytner, Steegmaier, Zöllner, Vestweber: "The binding of T cell-expressed P-selectin glycoprotein ligand-1 to E- and P-selectin is differentially regulated." dans: The Journal of biological chemistry, Vol. 272, Issue 45, pp. 28786-92, (1997) (PubMed).

Eppihimer, Wolitzky, Anderson, Labow, Granger: "Heterogeneity of expression of E- and P-selectins in vivo." dans: Circulation research, Vol. 79, Issue 3, pp. 560-9, (1996) (PubMed).

Ley, Bullard, Arbonés, Bosse, Vestweber, Tedder, Beaudet: "Sequential contribution of L- and P-selectin to leukocyte rolling in vivo." dans: The Journal of experimental medicine, Vol. 181, Issue 2, pp. 669-75, (1995) (PubMed).

Détails sur Selectin E/CD62e

Antigène: Selectin E/CD62e (SELE) (Selectin E (SELE))

Autre désignation: CD62E (SELE Produits)

Synonymes: anticorps SELE, anticorps CD62E, anticorps ELAM, anticorps ELAM1, anticorps ESEL, anticorps LECAM2, anticorps E-selectin, anticorps Elam, anticorps selectin E, anticorps selectin, endothelial cell, anticorps SELE, anticorps Sele

Sujet: Synonyms: E-selectin, ELAM-1

Pathways: Thromboxane A2 Receptor Signaling