CD8B anticorps

N° du produit ABIN2689439

Cet anticorps Souris Monoclonal détecte spécifiquement CD8B dans FACS, IP, ELISA, IHC (fro), CyTox et IHC (p). Il présente une réactivité envers Porc et a été mentionné dans 10+ publications.

Aperçu rapide pour CD8B anticorps (ABIN2689439)

Antigène: CD8B (CD8b Molecule (CD8B))

Reactivité: Porc

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD8B est non-conjugé

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Cytotoxicity Test (CyTox), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 295-33

Détail du produit anti-CD8B anticorps

Marque: BD Pharmingen™

Attributs du produit: The 295/33-25 antibody reacts with an epitope on the CD8 α chain, a 35 kDa antigen expressed on thymocytes, peripheral T lymphocytes, and NK cells. The CD8 Molecule can exist as a 70 kDa homodimer, composed of α chains, or heterodimer, composed of an α and a β chain. Two peripheral CD8+ T-cell populations can be distinguished in the pig: CD8-bright CD4- CTL effectors/precursors and CD8-dull CD4+ T-helper lymphocytes. Pig NK cells express CD8 (dull staining), CD2, MHC class II, LFA-1, and asialo-GM1, but not CD3, CD4, CD5, or CD6. mAb 295/33-25 was clustered as anti-CD8b at the First International Swine CD workshop. CD8 expression on peripheral blood lymphocytes. Yorkshire pig whole blood was stained with purified 295/33-25 monoclonal antibody followed by FITC-conjugated anti-mouse IgG2a mAb R19-15 (Cat. No. 553390), then PE-conjugated mAb 74-12-4 (anti-pig CD4a, Cat. No. 559586). Erythrocytes were lysed (BD PharmLyse ™, Cat. No. 555899), non-viable leukocytes were excluded by staining with 7-AAD (BD Via-Probe™, Cat. No. 555816/555815), and lymphocytes were gated according to scatter profile. Flow cytometry was performed on a BD FACSCalibur™

BD Pharmingen™ Purified Mouse Anti-Pig CD8b - Purified - Clone 295/33-25 - Isotype Mouse IgG2a, κ - Reactivity Pig - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Pig thymocytes

Isotype: IgG2a kappa

Information d'application

Indications d'application: Flow Cytometry System.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-CD8B (ABIN2689439)

Saalmüller, Werner, Fachinger: "T-helper cells from naive to committed." dans: Veterinary immunology and immunopathology, Vol. 87, Issue 3-4, pp. 137-45, (2002) (PubMed).

Zuckermann: "Extrathymic CD4/CD8 double positive T cells." dans: Veterinary immunology and immunopathology, Vol. 72, Issue 1-2, pp. 55-66, (2000) (PubMed).

Saalmüller, Pauly, Höhlich, Pfaff: "Characterization of porcine T lymphocytes and their immune response against viral antigens." dans: Journal of biotechnology, Vol. 73, Issue 2-3, pp. 223-33, (1999) (PubMed).

Zuckermann, Pescovitz, Aasted, Dominguez, Trebichavsky, Novikov, Valpotic, Nielsen, Arn, Sachs, Lunney, Boyd, Walker, Lee, Davis, Barbosa, Saalmüller: "Report on the analyses of mAb reactive with porcine CD8 for the second international swine CD workshop." dans: Veterinary immunology and immunopathology, Vol. 60, Issue 3-4, pp. 291-303, (1998) (PubMed).

Saalmüller, Aasted, Canals, Dominguez, Goldman, Lunney, Maurer, Pescovitz, Pospisil, Salmon: "Analyses of mAb reactive with porcine CD8." dans: Veterinary immunology and immunopathology, Vol. 43, Issue 1-3, pp. 249-54, (1995) (PubMed).

Saalmüller, Hirt, Maurer, Weiland: "Discrimination between two subsets of porcine CD8+ cytolytic T lymphocytes by the expression of CD5 antigen." dans: Immunology, Vol. 81, Issue 4, pp. 578-83, (1994) (PubMed).

Dato, Kim: "Characterization and utilization of a monoclonal antibody inhibiting porcine natural killer cell activity for isolation of natural killer and killer cells." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 11, pp. 4452-62, (1990) (PubMed).

Pescovitz, Lowman, Sachs: "Expression of T-cell associated antigens by porcine natural killer cells." dans: Immunology, Vol. 65, Issue 2, pp. 267-71, (1989) (PubMed).

Jonjić, Jonjić, Saalmüller, Rukavina, Koszinowski: "Distribution of T-lymphocyte subsets in porcine lymphoid tissues." dans: Immunology, Vol. 60, Issue 3, pp. 395-401, (1987) (PubMed).

Jonjić, Koszinowski: "Monoclonal antibodies reactive with swine lymphocytes. I. Antibodies to membrane structures that define the cytolytic T lymphocyte subset in the swine." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 133, Issue 2, pp. 647-52, (1984) (PubMed).

Détails sur CD8B

Antigène: CD8B (CD8b Molecule (CD8B))

Autre désignation: CD8b

Pathways: TCR Signaling