IL12 anticorps

N° du produit ABIN2689731

Il existe 2+ publications pour ce produit. L’anticorps anti-IL12 Monoclonal Rat est utilisé pour la détection de IL12 dans des échantillons de Souris. Il a été validé pour InhibAb.

Aperçu rapide pour IL12 anticorps (ABIN2689731)

Antigène: IL12 (Interleukin 12 (IL12))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp IL12 est non-conjugé

Application: Blocking Antibody (InhibAb)

Clone: C15-6

Détail du produit anti-IL12 anticorps

Attributs du produit: The C15.6 antibody reacts with both free and complexed (homodimer p80 and heterodimer p70) forms of the p40 subunit of mouse interleukin-12 (IL-12). The immunogen used to generate the C15.6 hybridoma was recombinant mouse IL-12 p70 protein. p40 has also been described as a subunit of IL-23 and thus it is possible that the C15.6 antibody will crossreact with IL-23. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Expression of IL-12 by mouse bone marrow-derived macrophages. Bone marrow cells from 6 month old BALB/c mice were cultured for 10 days in mouse GM-CSF (40 ng/mL final concentration, Cat. No. 554586). Adherent cells were washed and treated for ~14 hours with mouse IFN-γ (10 ng/mL final concentration, Cat. No. 554587), subsequently LPS (1 μg/mL final concentration, Sigma) and GolgiStop™ (2 μM final concentration Cat. No. 554724) were added to cultures for an additional 5 hours. Adherent cells were washed and then incubated with 1x trypsin EDTA at 37 °C. for 15 minutes and gently dislodged by pipetting. Nonspecific surface binding was blocked by incubation of cells with purified polyclonal normal mouse immunoglobulin. Cells were then fixed, permeabilized, and non-specific binding to intracellular antigens was blocked using PBS/2 % BSA/0.1 % saponin. Cells were then stained with 0.06 μg of PE-conjugated rat anti-mouse IL-12 (p40/p70) antibody (PE-C15.6, Cat. No. 554479, see left panel) using the BD Pharmingen™ staining protocol. To demonstrate specificity of staining, the binding of PE-C15.6 antibody was blocked by the preincubation of the conjugated antibody with recombinant mouse IL-12 p40 protein (0.5 μg, Cat. No. 554594, see middle panel), and by preincubating the fixed/permeabilized cells with unlabeled C15.6 antibody (5 μg/mL final concentration, Cat. No. 554477, see right panel) prior to staining. The quadrant markers for the bivariate dot plots were set based on the autofluorescence control and verified using the ligand blocking and unlabeled antibody blocking controls.

BD Pharmingen™ Purified Rat Anti-Mouse IL-12 (p40/p70) - Purified - Clone C15.6 - Isotype Rat IgG1 - Reactivity Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: CHO-expressed recombinant mouse IL-12 p70 protein

Isotype: IgG1

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-IL12 (ABIN2689731)

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." dans: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).

Wysocka, Kubin, Vieira, Ozmen, Garotta, Scott, Trinchieri: "Interleukin-12 is required for interferon-gamma production and lethality in lipopolysaccharide-induced shock in mice." dans: European journal of immunology, Vol. 25, Issue 3, pp. 672-6, (1995) (PubMed).

Détails sur IL12

Antigène: IL12 (Interleukin 12 (IL12))

Autre désignation: IL-12 p40,p70

Pathways: Signalistation JAK/STAT, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome