ARAF anticorps
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- Antigène Voir toutes ARAF Anticorps
- ARAF (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF))
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Reactivité
- Humain, Souris, Rat
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp ARAF est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificité
- This antibody detects endogenous levels of A-RAF protein. (region surrounding Asp297)
- Réactivité croisée (Details)
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. - Purification
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
- Pureté
- > 95 % pure by SDS-PAGE
- Top Product
- Discover our top product ARAF Anticorps primaire
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- Indications d'application
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ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1,0 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- DO NOT FREEZE!
- Stock
- 4 °C
- Stockage commentaire
- Store the antibody undiluted at 2-8 °C.
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- Antigène
- ARAF (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF))
- Autre désignation
- ARAF (ARAF Produits)
- Synonymes
- anticorps zgc:92074, anticorps wu:fk45h07, anticorps ARAF, anticorps a-raf, anticorps araf, anticorps araf1, anticorps pks2, anticorps rafa1, anticorps A-RAF, anticorps ARAF1, anticorps PKS2, anticorps RAFA1, anticorps 1200013E08Rik, anticorps A-Raf, anticorps AW495444, anticorps Araf1, anticorps A-Raf proto-oncogene, serine/threonine kinase, anticorps A-Raf proto-oncogene, serine/threonine kinase L homeolog, anticorps A-Raf proto-oncogene, serine/threonine kinase S homeolog, anticorps Araf proto-oncogene, serine/threonine kinase, anticorps araf, anticorps ARAF, anticorps araf.L, anticorps araf.S, anticorps Araf
- Sujet
- Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. A-Raf, a second member of the Raf gene family of serine/ threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of A-Raf is found at highest levels in urogenital tissues and kidney and at lowest level in brain tissue.Synonyms: A-Raf proto-oncogene serine/threonine-protein kinase, A-raf-1, ARAF1, PKS, PKS2, Proto-oncogene Pks
- Poids moléculaire
- approx. 68 kDa
- ID gène
- 369
- NCBI Accession
- NP_001645
- UniProt
- P10398
- Pathways
- Signalisation MAPK, Signalisation RTK, Hepatitis C
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