SUPT3H/SPT3 anticorps

N° du produit ABIN272144

Détail du produit anti-SUPT3H/SPT3 anticorps

Antigène: SUPT3H/SPT3 (SUPT3H) (Suppressor of Ty 3 Homolog (SUPT3H))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SUPT3H/SPT3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificité: This antibody detects endogenous levels of SPT3 protein. (region surrounding Glu203)

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Pureté: > 95 % pure by SDS-PAGE

Information d'application

Indications d'application: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: DO NOT FREEZE!

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C.

Détails sur SUPT3H/SPT3

Antigène: SUPT3H/SPT3 (SUPT3H) (Suppressor of Ty 3 Homolog (SUPT3H))

Autre désignation: SUPT3H / SPT3 (SUPT3H Produits)

Synonymes: anticorps SPT3, anticorps SPT3L, anticorps supt3h, anticorps MGC75923, anticorps zgc:92246, anticorps SUPT3H, anticorps SPT3 homolog, SAGA and STAGA complex component, anticorps SPT3 homolog, SAGA and STAGA complex component L homeolog, anticorps SUPT3H, anticorps supt3h, anticorps supt3h.L, anticorps Supt3h

Sujet: The transcription of many RNA polymerase II-dependent genes requires Spt3, a member of the S. cerevisiae SAGA complex. Transcription from δ sequences, the long terminal repeats that flank yeast Ty elements, requires the yeast SPT3 gene. Spt3 and Spt20 work together to recruit TATA-box binding protein (TBP) to the core promoter allowing TBP to bind to SAGA-dependent promoters. Null mutations in the Spt3 gene cause defects in sporulation, diploid filamentous growth, and haploid invasive growth, indicating that Spt3 has an important role in both mating and development pathways in yeast. At the promoters of some genes including yeast HO, HIS3 and TRP3 genes, Spt3 inhibits binding of TBP, resulting in reduced transcription. This repressive effect of Spt3 can be overcome by another member of the SAGA complex, GCN5, which promotes the formation of a TBP/TFIIA complex by histone acetylation.Synonyms: SPT3-like protein, SPT3L, Transcription initiation protein SPT3 homolog

Poids moléculaire: approx. 50 kDa

ID gène: 8464

NCBI Accession: NP_003590

UniProt: O75486