Bcl-2 anticorps (AA 41-54)

N° du produit ABIN2738523

L’anticorps anti-Bcl-2 Monoclonal Souris est utilisé pour la détection de Bcl-2 dans des échantillons de Humain, Porc, Singe, Chien et Chimpanzé. Il a été validé pour WB, IHC, IF, FACS, ELISA, IP et IHC (p).

Aperçu rapide pour Bcl-2 anticorps (AA 41-54) (ABIN2738523)

Antigène: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Reactivité: Humain, Porc, Singe, Chien, Chimpanzé

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Bcl-2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 8C8

Détail du produit anti-Bcl-2 anticorps

Épitope: AA 41-54

Specificité: Human BCL2 / Bcl-2

Aucune reactivité croisée: Souris, Rat (Rattus)

Purification: Protein G purified

Immunogène: A synthetic peptide, aa41-54 (GaaPAPGIFSSQPG-Cys) of human Bcl-2 protein. Percent identity by BLAST analysis: Human, Chimpanzee, Cat, Pig, Dog (100%), Gorilla, Orangutan, Bovine, Goat (91%), Galago (82%).

Type of Immunogen: Synthetic peptide

Isotype: IgG1 kappa

Information d'application

Indications d'application: Approved: ELISA, Flo (0.5 - 1 μg/10 x10⁶), IF (1 - 2 μg/mL), IHC, IHC-P (0.5 - 1 μg/mL), IP, WB (0.5 - 1 μg/mL)

Usage: This antibody recognizes a protein of 25-26  kDa, identified as the Bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of Bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of Bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express Bcl-2 protein and the small number in which the neoplastic cells are Bcl-2 negative. ELISA: Order BSA-free format for coating.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 mg/mL BSA, 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store at 2°C to 8°C.

Détails sur Bcl-2

Antigène: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Autre désignation: BCL2 / Bcl-2

Sujet: Name/Gene ID: BCL2
Family: Apoptosis

Synonyms: BCL2, Apoptosis regulator Bcl-2, B-cell CLL/lymphoma 2, B-cell lymphoma protein 2, Bcl-2, PPP1R50

ID gène: 596

Pathways: Signalisation MAPK, Signalisation PI3K-Akt, Apoptose, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling