APAF1 anticorps (N-Term)

N° du produit ABIN2745509

Cet anticorps Rat Monoclonal détecte spécifiquement APAF1 dans WB, ELISA, IP et ICC. Il présente une réactivité avec des échantillons de Souris et Rat.

Aperçu rapide pour APAF1 anticorps (N-Term) (ABIN2745509)

Antigène: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Reactivité: Souris, Rat

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp APAF1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: 13F11

Détail du produit anti-APAF1 anticorps

Épitope: AA 1-97, N-Term

Specificité: Recognizes the CARD domain of mouse and rat Apaf-1.

Réactivité croisée: Souris, Rat (Rattus)

Aucune reactivité croisée: Humain

Réactivité croisée (Details): Does not cross-react with human Apaf-1.

Pureté: >95 % (SDS-PAGE)

niveau d'endotoxine: <0.1EU/μg purified protein (LAL test, Lonza).

Immunogène: Recombinant mouse Apaf-1 (aa 1-97) containing the N-terminal CARD domain.

Isotype: IgG2a

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: In PBS containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.

Date de péremption: 12 months

Détails sur APAF1

Antigène: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Autre désignation: Apaf-1

Sujet: Apaf-1 is a cytoplasmic protein that forms one of the central hubs in the apoptosis regulatory network. It contains (from the N terminal) a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome, mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.

UniProt: Q9EPV5

Pathways: Signalisation p53, Apoptose, Caspase Cascade in Apoptosis, Tube Formation, Positive Regulation of Endopeptidase Activity