APAF1 anticorps (N-Term)

N° du produit ABIN2745510

Il existe 3+ publications pour ce produit. L’anticorps anti-APAF1 Monoclonal Rat est utilisé pour la détection de APAF1 dans des échantillons de Humain et Souris. Il a été validé pour WB, ELISA, ICC et IP.

Aperçu rapide pour APAF1 anticorps (N-Term) (ABIN2745510)

Antigène: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Reactivité: Humain, Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp APAF1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunoprecipitation (IP)

Clone: 18H2

Détail du produit anti-APAF1 anticorps

Épitope: AA 1-97, N-Term

Fonction: anti-Apaf-1, mAb (18H2)

Specificité: Recognizes the CARD domain of human and mouse Apaf-1. Does not cross-react with rat Apaf-1.

Aucune reactivité croisée: Rat (Rattus)

Réactivité croisée (Details): Does not cross-react with rat Apaf-1.

Attributs du produit: Monoclonal Antibody. Recognizes the CARD domain of human and mouse Apaf-1. Does not cross-react with rat Apaf-1. Isotype: Rat IgG2akappa. Clone: 18H2. Applications: ELISA, ICC, IP, WB. Liquid. In PBS containing 0.02 % sodium azide. Apaf-1 is a cytoplasmic protein that forms one of the central hubs in the apoptosis regulatory network. It contains (from the N terminal) a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome, mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.

Pureté: >95 % (SDS-PAGE)

niveau d'endotoxine: <0.1EU/μg purified protein (LAL test, Lonza).

Immunogène: Recombinant mouse Apaf-1 (aa 1-97) containing the N-terminal CARD domain.

Isotype: IgG2a kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: In PBS containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: After opening, prepare aliquots and store at -20 °C.Avoid freeze/thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire:

+4°C

-20°C

Stable for at least 1 year after receipt when stored at -20°C.

Date de péremption: 12 months

Références pour anticorps anti-APAF1 (ABIN2745510)

Oladzad, Nikkhah, Hosseinkhani: "Optimization of Experimental Variables Influencing Apoptosome Biosensor in HEK293T Cells." dans: Sensors (Basel, Switzerland), Vol. 20, Issue 6, (2020) (PubMed).

Tashakor, H-Dehkordi, OConnell, Gomez Ganau, Gozalbes, Eriksson, Hosseinkhani, Fearnhead: "A new split-luciferase complementation assay identifies pentachlorophenol as an inhibitor of apoptosome formation." dans: FEBS open bio, Vol. 9, Issue 7, pp. 1194-1203, (2020) (PubMed).

Noori, Hosseini, Nikkhah, Hosseinkhani: "Apoptosome formation upon overexpression of native and truncated Apaf-1 in cell-free and cell-based systems." dans: Archives of biochemistry and biophysics, Vol. 642, pp. 46-51, (2019) (PubMed).

Détails sur APAF1

Antigène: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Autre désignation: Apaf-1

Sujet:

Alternate Names/Synonyms: Apoptosis Protease-activating Factor 1

Product Description: Apaf-1 is a cytoplasmic protein that forms one of the central hubs in the apoptosis regulatory network. It contains (from the N terminal) a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome, mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.

UniProt: O88879

Pathways: Signalisation p53, Apoptose, Caspase Cascade in Apoptosis, Tube Formation, Positive Regulation of Endopeptidase Activity