CD11b anticorps

N° du produit ABIN2749012

Cet anticorps anti-CD11b est un anticorps Souris Monoclonal détectant CD11b dans FACS, IP, IHC (fro), ICC et Func. Adapté pour Humain et Non-Human Primate. Ce Primary Antibody a été cité dans 3+ publications.

Aperçu rapide pour CD11b anticorps (ABIN2749012)

Antigène: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reactivité: Humain, Non-Human Primate

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD11b est non-conjugé

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Functional Studies (Func)

Clone: ICRF44

Détail du produit anti-CD11b anticorps

Fonction: Anti-Hu CD11b Purified Low Endotoxin

Specificité: The mouse monoclonal antibody ICRF44 recognizes an extracellular epitope of CD11b (Mac-1alpha), a 165-170 kDa type 1 transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells.

Réactivité croisée (Details): Human, Non-Human Primates

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

niveau d'endotoxine: Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Immunogène: Rheumatoid synovial cells and human monocytes.

Isotype: IgG1

Information d'application

Indications d'application: Functional application: Blocking of granulocyte activation.
Flow cytometry: Recommended dilution: 1-12 μg/mL

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-CD11b (ABIN2749012)

Tieu, Lee, Sun, Lejeune, Recinos, Ju, Spratt, Guo, Milewicz, Tilton, Brasier: "An adventitial IL-6/MCP1 amplification loop accelerates macrophage-mediated vascular inflammation leading to aortic dissection in mice." dans: The Journal of clinical investigation, Vol. 119, Issue 12, pp. 3637-51, (2009) (PubMed).

Masten, Olson, Tarleton, Rund, Schuyler, Mehran, Archibeque, Lipscomb: "Characterization of myeloid and plasmacytoid dendritic cells in human lung." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 11, pp. 7784-93, (2006) (PubMed).

Hua, Sakamoto, Nagaoka: "Inhibitory actions of glucosamine, a therapeutic agent for osteoarthritis, on the functions of neutrophils." dans: Journal of leukocyte biology, Vol. 71, Issue 4, pp. 632-40, (2002) (PubMed).

Détails sur CD11b

Antigène: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Autre désignation: CD11b

Sujet: Integrin subunit alpha M,CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18), expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.,Mac-1, Integrin alpha M, ITGAM, CR3A, MO1A, MAC1A

ID gène: 3684

UniProt: P11215

Pathways: Apoptose, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation