CD103 anticorps (APC)

N° du produit ABIN2749099

Détail du produit anti-CD103 anticorps (APC)

Antigène: CD103 (ITGAE) (Integrin, alpha E (Antigen CD103, Human Mucosal Lymphocyte Antigen 1, alpha Polypeptide) (ITGAE))

Reactivité: Humain, Non-Human Primate

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD103 est conjugé à/à la APC

Application: Flow Cytometry (FACS)

Specificité: The mouse monoclonal antibody Ber-ACT8 recognizes an extracellular epitope of CD103 (alpha E integrin), a type I transmembrane glycoprotein primarily found on intestinal intraepithelial lymphocytes.

Réactivité croisée (Details): Human, Non-Human Primates

Purification: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogène: HTLV-1 induced human T cell line MAPS16

Clone: Ber-ACT8

Isotype: IgG1 kappa

Information d'application

Indications d'application: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Commentaires:

The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Stockage

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-CD103 (APC) (ABIN2749099)

Zhang, Bertucci, Ramsey-Goldman, Burt, Datta et al.: "Regulatory T cell (Treg) subsets return in patients with refractory lupus following stem cell transplantation, and TGF-beta-producing CD8+ Treg cells are associated with immunological remission of ..." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 10, pp. 6346-58, (2009) (PubMed).

Kost, Holden, Mann: "Marginal zone B-cell lymphoma: a retrospective immunophenotypic analysis." dans: Cytometry. Part B, Clinical cytometry, Vol. 74, Issue 5, pp. 282-6, (2008) (PubMed).

Cardoso, Garlet, Moreira, Júnior, Rossi, Silva: "Characterization of CD4+CD25+ natural regulatory T cells in the inflammatory infiltrate of human chronic periodontitis." dans: Journal of leukocyte biology, Vol. 84, Issue 1, pp. 311-8, (2008) (PubMed).

León, Roy: "Isolation of human small bowel intraepithelial lymphocytes by annexin V-coated magnetic beads." dans: Laboratory investigation; a journal of technical methods and pathology, Vol. 84, Issue 6, pp. 804-9, (2004) (PubMed).

Sigmundsdottir, Johnston, Gudjonsson, Bjarnason, Valdimarsson: "Methotrexate markedly reduces the expression of vascular E-selectin, cutaneous lymphocyte-associated antigen and the numbers of mononuclear leucocytes in psoriatic skin." dans: Experimental dermatology, Vol. 13, Issue 7, pp. 426-34, (2004) (PubMed).

Brandes, Willimann, Lang, Nam, Jin, Brenner, Morita, Moser: "Flexible migration program regulates gamma delta T-cell involvement in humoral immunity." dans: Blood, Vol. 102, Issue 10, pp. 3693-701, (2003) (PubMed).

French, Cresswell, Wong, Seymour, Charnley, Kirby: "T cell adhesion and cytolysis of pancreatic cancer cells: a role for E-cadherin in immunotherapy?" dans: British journal of cancer, Vol. 87, Issue 9, pp. 1034-41, (2002) (PubMed).

Détails sur CD103

Antigène: CD103 (ITGAE) (Integrin, alpha E (Antigen CD103, Human Mucosal Lymphocyte Antigen 1, alpha Polypeptide) (ITGAE))

Autre désignation: CD103 (ITGAE Produits)

Synonymes: anticorps A530055J10, anticorps CD103, anticorps aM290, anticorps alpha-E1, anticorps alpha-M290, anticorps HUMINAE, anticorps integrin alpha E, epithelial-associated, anticorps integrin subunit alpha E, anticorps Itgae, anticorps ITGAE

Sujet: Integrin subunit alpha E,CD103 / integrin alphaE is an integrin subunit that is expressed on intraepithelial lymphocytes, epithelial dendritic cells, lamina propria-derived dendritic cells, a subpopulation of lamina propria T cells, a small subset of peripheral lymphocytes, namely T reg cells, and on activated and TGF-beta stimulated lymphocytes. CD103 is in mature form cleaved into two disulfide-linked chains (C-terminal 150 kDa chain and N-terminal 25 kDa chain). It heterodimerizes with integrin beta7 subunit to form alphaE/beta7 integrin (mucosal lymphocyte 1 antigen), which through binding E-cadherin mediates homing of lymphocytes to the intestinal epithelium, and, in addition to the role in adhesion, may serve as an accessory molecule for intraepithelial lymphocyte activation.,Integrin alpha E, ITGAE

ID gène: 3682

UniProt: P38570