TNFRSF8 anticorps (PE)

N° du produit ABIN2749181

Détail du produit anti-TNFRSF8 anticorps (PE)

Antigène: TNFRSF8 (Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TNFRSF8 est conjugé à/à la PE

Application: Flow Cytometry (FACS)

Specificité: The mouse monoclonal antibody Ber-H8 recognizes extracellular part of CD30 (Ki-1 antigen), a 105 kDa single chain glycoprotein expressed on Hodgkin's and Reed-Sternberg cells, it is also found in Burkitt's lymphomas, virus-infected T and B lymphocytes, and on normal B and T lymphocytes after activation (T lymphocytes that produce Th2-type cytokines and on CD4+/CD8+ T lymphocytes that co-express CD45RO and the IL4 receptor).

Réactivité croisée (Details): Human

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Clone: Ber-H8

Isotype: IgG1 kappa

Information d'application

Indications d'application: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Commentaires:

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Stockage

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-TNFRSF8 (PE) (ABIN2749181)

Aalberse, Kapitein, de Roock, Klein, de Jager, van der Zee, Hoekstra, van Wijk, Prakken: "Cord blood CD4+ T cells respond to self heat shock protein 60 (HSP60)." dans: PLoS ONE, Vol. 6, Issue 9, pp. e24119, (2011) (PubMed).

Berro, Perry, Agrawal: "Increased expression and activation of CD30 induce apoptosis in human blood eosinophils." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 173, Issue 3, pp. 2174-83, (2004) (PubMed).

Matsumoto, Terakawa, Miura, Fukuda, Nakajima, Saito: "Extremely rapid and intense induction of apoptosis in human eosinophils by anti-CD30 antibody treatment in vitro." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 4, pp. 2186-93, (2004) (PubMed).

Franke, Jung, Ellis: "Characterization of the CD30L binding domain on the human CD30 molecule using anti-CD30 antibodies." dans: Hybridoma, Vol. 19, Issue 1, pp. 43-8, (2000) (PubMed).

Falini, Pileri, Pizzolo, Dürkop, Flenghi, Stirpe, Martelli, Stein: "CD30 (Ki-1) molecule: a new cytokine receptor of the tumor necrosis factor receptor superfamily as a tool for diagnosis and immunotherapy." dans: Blood, Vol. 85, Issue 1, pp. 1-14, (1995) (PubMed).

Détails sur TNFRSF8

Antigène: TNFRSF8 (Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8))

Autre désignation: CD30 (TNFRSF8 Produits)

Synonymes: anticorps CD30, anticorps D1S166E, anticorps Ki-1, anticorps Cd30, anticorps Ki, anticorps TNFRSF8, anticorps TNF receptor superfamily member 8, anticorps tumor necrosis factor receptor superfamily, member 8, anticorps TNFRSF8, anticorps Tnfrsf8

Sujet: TNF receptor superfamily member 8,CD30 is a type I transmembrane glycoprotein of the TNF receptor superfamily. CD30 was originally identified as a cell surface antigen of Hodgkins and Reed-Sternberg cells using monoclonal antibody Ki-1. The ligand for CD30 is CD30L (CD153). The binding of CD30 to CD30L mediates pleiotropic effects including cell proliferation, activation, differentiation, and apoptotic cell death. CD30 has a critical role in the pathophysiology of Hodgkin's disease and other CD30+ lymphomas. CD30 acts as a costimulatory molecule in thymic negative selection. In addition to its expression on Hodgkin's and Reed-Sternberg cells, CD30 is also found in some non-Hodgkin's lymphomas (including Burkitt's lymphomas), virus-infected T and B cells, and on normal T and B cells after activation. In T cells, CD30 expression is present on a subset of T cells that produce Th2-type cytokines and on CD4+/CD8+ thymocytes that co-express CD45RO and the IL4 receptor. Soluble form of CD30 (sCD30) serves as a marker reflecting Th2 immune response.,Ki-1, TNFRSF8, CD30L receptor, D1S166E

ID gène: 943

UniProt: P28908