GCLC anticorps (N-Term)

N° du produit ABIN2774078

L’anticorps Lapin Polyclonal anti-GCLC a été validé pour WB et IHC. Il convient pour détecter GCLC dans des échantillons de Humain, Souris, Rat, Boeuf (Vache), Poisson zèbre (Danio rerio), Lapin, Chien, Cobaye et Cheval. Il y a 1 publication disponible.

Aperçu rapide pour GCLC anticorps (N-Term) (ABIN2774078)

Antigène: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Poisson zèbre (Danio rerio), Lapin, Chien, Cobaye, Cheval

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GCLC est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-GCLC anticorps

Épitope: N-Term

Séquence: VLETLQEKGE RTNPNHPTLW RPEYGSYMIE GTPGQPYGGT MSEFNTVEAN

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 79%

Attributs du produit: This is a rabbit polyclonal antibody against GCLC. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogène: The immunogen is a synthetic peptide directed towards the N terminal region of human GCLC

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator.

Commentaires:

Antigen size: 637 AA

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Références pour anticorps anti-GCLC (ABIN2774078)

Tomasi, Ryoo, Yang, Iglesias Ara, Ko, Lu: "Molecular mechanisms of lipopolysaccharide-mediated inhibition of glutathione synthesis in mice." dans: Free radical biology & medicine, Vol. 68, pp. 148-58, (2014) (PubMed).

Détails sur GCLC

Antigène: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Autre désignation: GCLC

Sujet: Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. GCLC is the catalytic subunit of 637 amino acids with a calculated molecular weight of 72.773 kDa. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The gene encoding the catalytic subunit encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kDa and maps to chromosome 6. The regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: GCS, GLCL, GLCLC, GCL
Protein Interaction Partner: UBC, PPID, GCLM, CCL22, PAXIP1, ELAVL1,
Protein Size: 637

Poids moléculaire: 73 kDa

ID gène: 2729

NCBI Accession: NM_001498, NP_001489

UniProt: P48506

Pathways: Cell RedoxHomeostasis