LIG4 anticorps (N-Term)

N° du produit ABIN2778272

Détail du produit anti-LIG4 anticorps

Antigène: LIG4 (Ligase IV, DNA, ATP-Dependent (LIG4))

Épitope: N-Term

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien, Cheval, Lapin, Cobaye

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp LIG4 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Séquence: DGERMQMHKD GDVYKYFSRN GYNYTDQFGA SPTEGSLTPF IHNAFKADIQ

Homologie: Cow: 92%, Dog: 93%, Guinea Pig: 93%, Horse: 86%, Human: 100%, Mouse: 92%, Rabbit: 93%, Rat: 93%

Attributs du produit: This is a rabbit polyclonal antibody against LIG4. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogène: The immunogen is a synthetic peptide directed towards the N terminal region of human LIG4

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator.

Commentaires:

Antigen size: 911 AA

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Détails sur LIG4

Antigène: LIG4 (Ligase IV, DNA, ATP-Dependent (LIG4))

Autre désignation: LIG4 (LIG4 Produits)

Synonymes: anticorps zgc:165595, anticorps lig4, anticorps DNA LIGASE IV, anticorps LIG4, anticorps lig4-A, anticorps 5830471N16Rik, anticorps tiny, anticorps DNL4, anticorps ligase IV, DNA, ATP-dependent, anticorps DNA ligase 4, anticorps ATP-dependent DNA ligase implicated in dsDNA break repair via nonhomologous end-joining, anticorps DNA ligase IV, anticorps ligase IV, DNA, ATP-dependent L homeolog, anticorps lig4, anticorps LIG4, anticorps LOC100212302, anticorps CpipJ_CPIJ005161, anticorps PTRG_09982, anticorps MCYG_05151, anticorps LOC657210, anticorps ATLIG4, anticorps lig4.L, anticorps Lig4

Sujet: LIG4 encodes a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome.
Protein Interaction Partner: XRCC4, UBC, AGO2, GZMK, ZSCAN18, PHF10, PNKP, TSFM, PRKDC, GUSBP3, NOMO3, FBLL1, PGP, ASPM, AMZ2P1, PPP1R18, COMMD1, ZNF428, AGAP4, WDR20, RPS19BP1, ZFAND2A, FIP1L1, CCDC176, VKORC1, COA7, SOWAHC, SEMA4G, ALMS1, WARS, UBE2L3, UBB, TPT1, TPI1, TP53BP1, TLE
Protein Size: 911

Poids moléculaire: 104 kDa

ID gène: 3981

NCBI Accession: NM_002312, NP_002303

UniProt: Q8IY66

Pathways: Réparation de l'ADN, Production of Molecular Mediator of Immune Response