POLR3A anticorps (Middle Region)

N° du produit ABIN2783481

Détail du produit anti-POLR3A anticorps

Antigène: POLR3A (Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A))

Épitope: Middle Region

Reactivité: Humain, Souris, Rat, Chien, Lapin, Boeuf (Vache), Cheval, Cobaye, Saccharomyces cerevisiae

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp POLR3A est non-conjugé

Application: Western Blotting (WB)

Séquence: AYFGQKDSVC GVSECIIMGI PMNIGTGLFK LLHKADRDPN PPKRPLIFDT

Homologie: Cow: 79%, Dog: 86%, Guinea Pig: 86%, Horse: 79%, Human: 100%, Mouse: 79%, Rabbit: 86%, Rat: 86%, Yeast: 85%

Attributs du produit: This is a rabbit polyclonal antibody against POLR3A. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogène: The immunogen is a synthetic peptide directed towards the middle region of human POLR3A

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator.

Commentaires:

Antigen size: 1390 AA

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Détails sur POLR3A

Antigène: POLR3A (Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A))

Autre désignation: POLR3A (POLR3A Produits)

Synonymes: anticorps 9330175N20Rik, anticorps BC053071, anticorps RPC1, anticorps RPC155, anticorps RGD1305574, anticorps ADDH, anticorps HLD7, anticorps hRPC155, anticorps polymerase (RNA) III (DNA directed) polypeptide A, anticorps RNA polymerase III subunit A, anticorps Polr3a, anticorps POLR3A

Sujet: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. POLR3A is the largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. It forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition.
Alias Symbols: RPC1, RPC155, hRPC155, ADDH, HLD7
Protein Interaction Partner: SUMO2, UBC, ASB13, NEDD4, POLR3H, VPS18, POLR3B, POLR1D, CRCP, POLR1A, POLR3C, POLR1C, SMARCA5, POLR2H, POLR2E, POLR3D, IKBKG, PCBD1, EWSR1, RPAP2, RPAP3, RPAP1, GPN1, RAE1, POLR3E, PKP2, GTF2B,
Protein Size: 1390

Poids moléculaire: 156 kDa

ID gène: 11128

NCBI Accession: NM_007055, NP_008986

UniProt: O14802