Crossover junction endonuclease EME1 (EME1) (Middle Region) anticorps

N° du produit ABIN2785184

Détail du produit

Antigène: Crossover junction endonuclease EME1 (EME1)

Épitope: Middle Region

Reactivité: Humain, Souris, Rat, Porc, Lapin, Boeuf (Vache), Chien, Cobaye, Cheval

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Western Blotting (WB)

Séquence: AYPSPQLLVQ AYQQCFSDKE RQNLLADIQV RRGEGVTSTS RRIGPELSRR

Homologie: Cow: 93%, Dog: 100%, Guinea Pig: 79%, Horse: 93%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 93%

Attributs du produit: This is a rabbit polyclonal antibody against EME1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogène: The immunogen is a synthetic peptide directed towards the middle region of human EME1

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator.

Commentaires:

Antigen size: 570 AA

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Détail du antigène

Antigène: Crossover junction endonuclease EME1 (EME1)

Autre désignation: EME1 (EME1 Produits)

Synonymes: anticorps 6820428D13, anticorps essential meiotic structure-specific endonuclease 1, anticorps Eme1

Sujet: EME1 interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. EME1 may be required in mitosis for the processing of stalled or collapsed replication forks.EME1 and MUS81 (MIM 606591) form an endonuclease complex that cleaves branched DNA structures, especially those arising during stalled DNA replication (Abraham et al., 2003 [PubMed 14609959]).[supplied by OMIM].
Alias Symbols: FLJ31364, MMS4L, SLX2A
Protein Interaction Partner: vpr, MUS81, APP, SLX1A, EME1, SLX4, SUMO2, SLX1B,
Protein Size: 570

Poids moléculaire: 63 kDa

ID gène: 146956

NCBI Accession: NM_152463, NP_689676

UniProt: Q96AY2