TGFB1 anticorps (Biotin)

N° du produit ABIN290963

L’anticorps anti-TGFB1 Polyclonal Poulet est utilisé pour la détection de TGFB1 dans des échantillons de Humain et Souris. Il a été validé pour WB et ELISA.

Aperçu rapide pour TGFB1 anticorps (Biotin) (ABIN290963)

Antigène: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Reactivité: Humain, Souris

Hôte: Poulet

Clonalité: Polyclonal

Conjugué: Cet anticorp TGFB1 est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA

Détail du produit anti-TGFB1 anticorps (Biotin)

Specificité: Recognizes human TGFb1. When used in combination with the monoclonal capture antibody LS-C10854 in a sandwich ELISA, ~5 % cross-reactivity with TGF-b1.2 and 1 % cross-reactivity with rhTGF-b2, rcTGF-b3 and raTG-b5 was observed.

Purification: Affinity purified

Immunogène: Recombinant human TGF-b1 expressed in CHO cells.

Type of Immunogen: Recombinant protein

Isotype: IgY

Information d'application

Indications d'application: Approved: ELISA, WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in ELISA and Western Blot. Plates are coated with 100 μL/well of the capture antibody in combination with 100 μL/well of the detection antibody at 300 ng/mL. An ELISA for sample volumes of 100 μL can be obtained. Titrate each preparation of the recombinant protein for standard preparation to arrive at the most suitable dose range. For this antibody pair, a two-fold dilution series starting at 2 ng/mL is suggested. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: Sterile PBS. Resuspend to 250 μL

Concentration: Lot specific

Buffer: Supplied as a lyopholized powder from PBS,  pH 7.4, 50 μg BSA.

Conseil sur la manipulation: Avoid frequent freeze thaws. Store undiluted.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur TGFB1

Antigène: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Autre désignation: TGFB1 / TGF Beta 1

Sujet: Name/Gene ID: TGFB1
Family: TGF beta

Synonyms: TGFB1, CED, DPD1, Diaphyseal dysplasia 1, LAP, Latency-associated peptide, TGF Beta, TGFbeta, TGF-beta 1 protein, TGF-beta-1, TGFB

ID gène: 7040

UniProt: P01137

Pathways: EGFR Signaling Pathway, Dopaminergic Neurogenesis, Cellular Response to Molecule of Bacterial Origin, Glycosaminoglycan Metabolic Process, Regulation of Leukocyte Mediated Immunity, Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Ribonucleoside Biosynthetic Process, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy, Cancer Immune Checkpoints