TGFB1 anticorps

N° du produit ABIN465317

L’anticorps anti-TGFB1 Monoclonal Souris est utilisé pour la détection de TGFB1 dans des échantillons de Humain, Souris et Singe. Il a été validé pour WB et ELISA.

Aperçu rapide pour TGFB1 anticorps (ABIN465317)

Antigène: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Reactivité: Humain, Souris, Singe

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TGFB1 est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-TGFB1 anticorps

Specificité: Human TGF-beta1

Purification: Protein G purified

Immunogène: Hi-5 Insect cell derived recombinant Human TGF-beta1.

Isotype: IgG1

Information d'application

Indications d'application: Approved: ELISA, WB

Usage: Immunohistochemistry: This antibody stained formalin-fixed paraffin-embedded sections of human pancreas adenocarcinoma tissue. The recommended concentration is 5 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with an alcohol-soluble AEC chromogen. Heat induced antigen retrieval with a  pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. *Additional Immunostaining data available. Please contact Tech Support for information. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 4-8 μg/mL of this antibody will detect at least 100 pg/mL of recombinant Human TGF-beta1 when used in conjunction with compatible secondary reagents. Western Blot: To detect Human TGF-beta1 by Western Blot analysis this antibody can be used at a concentration of 2-4 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human TGF-beta1 is 2-4 ng/lane, under non-reducing conditions. In a dot blot, a 1:500 dilution of this antibody exhibited complete cross reactivity with Human TGF-beta2 and TGF-beta3 isotypes. Reduced cross reactivity was shown at a 1:1000 dilution.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS

Conseil sur la manipulation: Avoid freeze/thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Détails sur TGFB1

Antigène: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Autre désignation: TGFB1 / TGF Beta 1

Sujet: Name/Gene ID: TGFB1
Family: TGF beta

Synonyms: TGFB1, CED, DPD1, Diaphyseal dysplasia 1, LAP, Latency-associated peptide, TGF Beta, TGFbeta, TGF-beta 1 protein, TGF-beta-1, TGFB

ID gène: 7040

UniProt: P01137

Pathways: EGFR Signaling Pathway, Dopaminergic Neurogenesis, Cellular Response to Molecule of Bacterial Origin, Glycosaminoglycan Metabolic Process, Regulation of Leukocyte Mediated Immunity, Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Ribonucleoside Biosynthetic Process, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy, Cancer Immune Checkpoints