Histone anticorps

N° du produit ABIN3024805

Détail du produit

Antigène: Histone

Reactivité: Humain, Rat, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (IF)

Purification: Protein G affinity chromatography

Immunogène: Nuclei of human leukemia biopsy cells were used as the immunogen for the Histone antibody.

Clone: AE-4

Isotype: IgG2a kappa

Information d'application

Indications d'application: Optimal dilution of the Histone antibody should be determined by the researcher.

1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 0.5-1 μg/mL, Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store the Histone antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détail du antigène

Antigène: Histone

Synonymes: anticorps Histone H2A, anticorps Histone H2B 1, anticorps Histone H3, anticorps Probable histone H2B 4, anticorps Histone H2B 2, anticorps Histone, anticorps his-16, anticorps his-12, anticorps his-11, anticorps his-9, anticorps his-43, anticorps his-61, anticorps his-48, anticorps his-57, anticorps his-22, anticorps his-4, anticorps his-3, anticorps his-42, anticorps his-66, anticorps his-65, anticorps Histone

Sujet: Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer, formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80 % of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.