ICAM-3/CD50 anticorps
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- Antigène Voir toutes ICAM-3/CD50 (ICAM3) Anticorps
- ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp ICAM-3/CD50 est non-conjugé
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Application
- Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purification
- Protein G affinity chromatography
- Immunogène
- Recombinant human protein was used as the immunogen for the ICAM3 antibody.
- Clone
- ICAM3-1019
- Isotype
- IgG2b kappa
- Top Product
- Discover our top product ICAM3 Anticorps primaire
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- Indications d'application
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Optimal dilution of the ICAM3 antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2) - Restrictions
- For Research Use only
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- Concentration
- 0.2 mg/mL
- Buffer
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Store the ICAM3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Antigène
- ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))
- Autre désignation
- ICAM3 (ICAM3 Produits)
- Synonymes
- anticorps CD50, anticorps CDW50, anticorps ICAM-R, anticorps ICAM-3, anticorps ICAM3, anticorps intercellular adhesion molecule 3, anticorps ICAM3, anticorps Icam3
- Sujet
- Recognizes an N-glycosylated glycoprotein of 120 kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This mAb is excellent for staining of formalin/paraffin tissues.
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