BAX anticorps

N° du produit ABIN3026588

Cet anticorps Souris Monoclonal détecte spécifiquement BAX dans WB, ELISA, IF, FACS, IP et IHC (p). Il présente une réactivité envers Humain et Singe.

Aperçu rapide pour BAX anticorps (ABIN3026588)

Antigène: BAX (BCL2-Associated X Protein (BAX))

Reactivité: Humain, Singe

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp BAX est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 2D2

Détail du produit anti-BAX anticorps

Aucune reactivité croisée: Souris, Rat (Rattus)

Attributs du produit: This antibody recognizes a protein of 21 kDa, identified as the Bax protein. The Bax antibody is highly specific and shows no cross-reaction with Bcl-2 alpha or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and the protein also counters the death repressor activity of Bcl-2.

Purification: Protein G affinity chromatography

Immunogène: Amino acids GSGEQPRGGGPTSS (3-16) were used as the immunogen for this anti-human Bax antibody.

Isotype: IgG1

Information d'application

Indications d'application: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Bax antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 1  mM EDTA buffer,  pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. ELISA: order BSA-free format for coating,FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,WB: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the Bax antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur BAX

Antigène: BAX (BCL2-Associated X Protein (BAX))

Autre désignation: Bax

Sujet: This antibody recognizes a protein of 21  kDa, identified as the Bax protein. The Bax antibody is highly specific and shows no cross-reaction with Bcl-2 alpha or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and the protein also counters the death repressor activity of Bcl-2.

ID gène: 581

Pathways: Signalisation p53, Signalisation PI3K-Akt, Apoptose, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response