Paxillin anticorps (AA 456-472)

N° du produit ABIN3032168

L’anticorps Lapin Polyclonal anti-Paxillin a été validé pour WB, ICC, IHC (p) et IHC (fro). Il convient pour détecter Paxillin dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour Paxillin anticorps (AA 456-472) (ABIN3032168)

Antigène: Paxillin (PXN)

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Paxillin est non-conjugé

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-Paxillin anticorps

Épitope: AA 456-472

Purification: Antigen affinity

Immunogène: Amino acids 456-472 (HEKDGKAYCRKDYFDMF) were used as the immunogen for this Paxillin antibody (100% homologous in human, mouse and rat).

Isotype: IgG

Information d'application

Indications d'application: The stated application concentrations are suggested starting amounts. Titration of the Paxillin antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,IHC (Frozen): 0.5-1 μg/mL,Immunocytochemistry: 0.5-1 μg/mL

Restrictions: For Research Use only

Stockage

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Stock: -20 °C

Stockage commentaire: After reconstitution, the Paxillin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Détails sur Paxillin

Antigène: Paxillin (PXN)

Autre désignation: Paxillin

Sujet: Paxillin is a signal transduction adaptor protein discovered in 1990 in the laboratory of Keith Burridge. Salgia et al.(1995) mapped the gene to 12q24 using fluorescence in situ hybridization.The C-terminal region of Paxillin contains four LIM domains that target paxillin to focal adhesions, it is presumed through a direct association with the cytoplasmic tail of beta-integrin. The N-terminal region is rich in protein-protein interaction sites. The proteins that bind to Paxillin are diverse and include protein tyrosine kinases, such as Src and FAK, structural proteins, such as vinculin and actopaxin, and regulators of actin organization, such as COOL/PIX and PKL/GIT. Paxillin is tyrosine-phosphorylated by FAK and Src upon integrin engagement or growth factor stimulation, creating binding sites for the adapter protein Crk. The protein contains 4 LIM domains, a proline-rich domain containing a consensus SH3-binding site, and 3 potential SH2-binding sites.

UniProt: P49023

Pathways: Signalisation MAPK, EGFR Signaling Pathway, Response to Growth Hormone Stimulus, Cell-Cell Junction Organization, Maintenance of Protein Location, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling