Cofilin anticorps (C-Term)

N° du produit ABIN3042746

L’anticorps Lapin Polyclonal anti-Cofilin a été validé pour WB, IHC, IF et ICC. Il convient pour détecter Cofilin dans des échantillons de Humain, Souris, Rat et Singe.

Aperçu rapide pour Cofilin anticorps (C-Term) (ABIN3042746)

Antigène: Cofilin (CFL1) (Cofilin 1 (CFL1))

Reactivité: Humain, Souris, Rat, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Cofilin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Détail du produit anti-Cofilin anticorps

Épitope: AA 122-152, C-Term

Fonction: Anti-Cofilin/CFL1 Antibody

Séquence: DAIKKKLTGI KHELQANCYE EVKDRCTLAE K

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-Cofilin/CFL1 Antibody (ABIN3042746). Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human Cofilin, identical to the related mouse and rat sequences.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human, Monkey, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur Cofilin

Antigène: Cofilin (CFL1) (Cofilin 1 (CFL1))

Autre désignation: CFL1

Sujet:

Synonyms: Cofilin-1,18 kDa phosphoprotein,p18,Cofilin, non-muscle isoform,CFL1,CFL,

Tissue Specificity: Widely distributed in various tissues.

Background: Cofilin, a member of the ADF/cofilin family, is actually a protein with 70 % sequence homology to ADF, making it part of the ADF/cofilin family of small ADP-binding proteins. It is a ubiquitous actin-binding factor required for the reorganization of actin filaments. The protein is known to sever actin filaments by creating more positive ends on filament fragments, it can cause depolymerization at the minus end of filaments, thereby preventing their reassembly. As a long-lasting in vivo effect, cofilin recycles older ADP-F-actin, helping cell to maintain ATP-G-actin pool for sustained motility. pH , phosphorylation and phosphoinositides regulate cofilin's binding and associating activity with actin.

Sequence Similarities: Contains 1 HMG box DNA-binding domain.

Poids moléculaire: 19 kDa

ID gène: 1072

UniProt: P23528

Pathways: Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus, CXCR4-mediated Signaling Events