Cofilin anticorps (AA 2-166)

N° du produit ABIN3042747

Cet anticorps anti-Cofilin est un anticorps Lapin Polyclonal détectant Cofilin dans WB, IHC, ELISA, IF, FACS, ICC et IHC (fro). Adapté pour Humain, Souris, Rat et Singe.

Aperçu rapide pour Cofilin anticorps (AA 2-166) (ABIN3042747)

Antigène: Cofilin (CFL1) (Cofilin 1 (CFL1))

Reactivité: Humain, Souris, Rat, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Cofilin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-Cofilin anticorps

Épitope: AA 2-166

Fonction: Anti-Cofilin/CFL1 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-Cofilin/CFL1 Antibody Picoband® (ABIN3042747). Tested in ELISA, Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human Cofilin recombinant protein (Position: A2-L166). Human Cofilin shares 99% amino acid (aa) sequences identity with both mouse and rat Cofilin.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat, Monkey
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Cooper, G. M. and R. E. Hausman. The Cell: A Molecular Approach, 3rd ed. Washington DC: ASM Press 2004 pp.436-440. 2. Ichetovkin I, Han J, Pang KM, Knecht DA, Condeelis JS (April 2000). "Actin filaments are severed by both native and recombinant dictyostelium cofilin but to different extents". Cell Motil. Cytoskeleton 45 (4): 293-306. 3. Lappalainen P, Drubin DG (July 1997). "Cofilin promotes rapid actin filament turnover in vivo".Nature 388 (6637): 78-82.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur Cofilin

Antigène: Cofilin (CFL1) (Cofilin 1 (CFL1))

Autre désignation: CFL1

Sujet:

Synonyms: Cofilin-1,18 kDa phosphoprotein,p18,Cofilin, non-muscle isoform,CFL1,CFL,

Tissue Specificity: Widely distributed in various tissues.

Background: Cofilin, a member of the ADF/cofilin family, is actually a protein with 70 % sequence homology to ADF, making it part of the ADF/cofilin family of small ADP-binding proteins. It is a ubiquitous actin-binding factor required for the reorganization of actin filaments. The protein is known to sever actin filaments by creating more positive ends on filament fragments, it can cause depolymerization at the minus end of filaments, thereby preventing their reassembly. As a long-lasting in vivo effect, cofilin recycles older ADP-F-actin, helping cell to maintain ATP-G-actin pool for sustained motility. pH , phosphorylation and phosphoinositides regulate cofilin's binding and associating activity with actin.

Sequence Similarities: Belongs to the actin-binding proteins ADF family.

Poids moléculaire: 19 kDa

ID gène: 1072

UniProt: P23528

Pathways: Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus, CXCR4-mediated Signaling Events